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In vitro diagnostic for the quantitative determination of carbohydrate-deficient transferrin (CDT) in human serum by means of particle-enhanced immunonephelometry using the BN™ II and BN ProSpec® System. The N Latex CDT assay must be run concurrently with the N Antisera to Human Transferrin assay so that the result can be expressed as a relative ratio, i.e., %CDT of the total transferrin. The calculation of %CDT can be used as a tool to identify possible chronic heavy alcohol consumption.

The CDT in the sample competes with CDT-coated polystyrene particles for the bond to specific monoclonal antibodies against human CDT, which are likewise bound to polystyrene particles. In the presence of CDT in the sample, there is no or little aggregation of the polystyrene particles. In the absence of CDT in the sample, the polystyrene particles aggregate. The higher the CDT content in the assay, the lower the scattered light signal. The evaluation is performed by comparison with a standard of known concentration.

The Axis ® %CDT Turbidimetric Immunoassay (TIA) is intended for the quantitative measurement of carbohydrate deficient transferrin (CDT) in human serum, as a tool to identify possible chronic heavy alcohol consumption. The Axis ® %CDT Turbidimetric Immunoassay is designed for the quantitative determination of carbohydrate deficient transferrin (CDT) in human serum.

The Axis® %CDT is a heterogeneous immunoassay with column separation followed by turbidimetric measurement. Serum transferrin in the sample is saturated with Fe ". The mixture is applied to an ion-exchange column. Due to the different amounts of sialic residues on transferrin, the isoforms carry different charges and are separated in the column. The CDT isoforms are eluted. The CDT content of the collected eluate is determined by turbidimetric measurement. The eluted CDT isoforms form immune complexes with anti-transferrin antibodies. Total transferrin content of the sample is determined separately, using the same anti- transferrin antibodies. The measurements are evaluated using a calibration curve, and the Axis® %CDT value is calculated.