K Number
K112790
Device Name
AXIS-SHIELD 3-REAGENT HOMOCYSTEINE ASSAY FOR SYNCHRON
Date Cleared
2012-05-07

(224 days)

Product Code
Regulation Number
862.1377
AI/MLSaMDIVD (In Vitro Diagnostic)TherapeuticDiagnosticis PCCP Authorized
Intended Use
The 3-Reagent Homocysteine Assay for Beckman Coulter SYNCHRON® and UniCel® systems is intended for in vitro quantitative determination of total homocysteine in human serum and plasma. The device can assist in the diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria.
Device Description
Bound or dimerised homocysteine (oxidised form) is reduced to free homocysteine, which then reacts with serine catalysed by cystathionine beta-synthase (CBS) to form cystathionine. Cystathionine in turn is broken down by cystathionine beta-lyase (CBL) to form homocysteine, pyruvate and ammonia. Pyruvate is then converted by lactate dehydrogenase (LDH) to lactate with nicotinamide adenine dinucleotide (NADH) as coenzyme. The rate of NADH conversion to NAD+ is directly proportional to the concentration of homocysteine (delta A340 nm).
More Information

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No
The device description details a chemical assay based on enzymatic reactions and spectrophotometric measurement, with no mention of AI or ML terms or concepts.

No
The device is an in vitro diagnostic (IVD) assay designed to quantitatively determine total homocysteine levels in human serum and plasma, assisting in diagnosis, rather than directly treating a condition.

Yes

Explanation: The "Intended Use / Indications for Use" section explicitly states, "The device can assist in the diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria." This directly indicates its role in diagnosis.

No

The device description clearly outlines a chemical assay involving reagents and reactions, which are physical components and processes, not solely software. The performance studies also focus on the analytical performance of the assay itself, not software functionality.

Yes, this device is an IVD (In Vitro Diagnostic).

Here's why:

  • Intended Use: The "Intended Use / Indications for Use" section explicitly states that the assay is "intended for in vitro quantitative determination of total homocysteine in human serum and plasma." The term "in vitro" is a key indicator of an IVD.
  • Sample Type: The assay analyzes "human serum and plasma," which are biological samples taken from the body but tested outside of it.
  • Purpose: The assay is used to "assist in the diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria," which is a diagnostic purpose.
  • Device Description: The description details a chemical reaction that occurs with the sample, which is typical of an in vitro diagnostic test.

N/A

Intended Use / Indications for Use

The 3-Reagent Homocysteine Assay for Beckman Coulter SYNCHRON® and UniCel® systems is intended for in vitro quantitative determination of total homocysteine in human serum and plasma. The device can assist in the diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria.

Product codes (comma separated list FDA assigned to the subject device)

LPS

Device Description

Bound or dimerised homocysteine (oxidised form) is reduced to free homocysteine, which then reacts with serine catalysed by cystathionine beta-synthase (CBS) to form cystathionine. Cystathionine in turn is broken down by cystathionine beta-lyase (CBL) to form homocysteine, pyruvate and ammonia. Pyruvate is then converted by lactate dehydrogenase (LDH) to lactate with nicotinamide adenine dinucleotide (NADH) as coenzyme. The rate of NADH conversion to NAD+ is directly proportional to the concentration of homocysteine (△ A340 nm).

Mentions image processing

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Mentions AI, DNN, or ML

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Input Imaging Modality

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Anatomical Site

Not Found

Indicated Patient Age Range

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Intended User / Care Setting

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Description of the training set, sample size, data source, and annotation protocol

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Description of the test set, sample size, data source, and annotation protocol

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Summary of Performance Studies (study type, sample size, AUC, MRMC, standalone performance, key results)

The 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® demonstrated substantially equivalent performance to the Axis-Shield Liquid Stable (LS) 2-Part Homocysteine Reagent as indicated by a method comparison study, in which a Passing & Bablock method comparison and a Pearson correlation analysis was conducted using 100 samples covering the full measuring range of the assay.

The 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® on the Synchron. LX Pro analyzer demonstrated substantially equivalent performance to the Axis-Shield Liquid Stable (LS) 2-Part Homocysteine Reagent on the Olympus AU400 analyser as indicated by a slope of 1.01 (95% Cl: 0.99 to 1.04), an intercept of 0.07 (95% Cl: -0.30 to 0.44) and a correlation coefficient (r) of 0.997 (95% CI: 0.99 to 1.00).

The 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® on the Unicel DxC analyzer demonstrated substantially equivalent performance to the Axis-Shield Liquid Stable (LS) 2-Part Homocysteine Reagent on the Olympus AU400 analyser as indicated by a slope of 0.99 (95% Cl: 0.97 to 1.02), an intercept of 0.74 (95% Cl: 0.30 to 1.11) and a correlation coefficient (r) of 0.994 (95% CI: 0.99 to 1.00).

Key Metrics (Sensitivity, Specificity, PPV, NPV, etc.)

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Predicate Device(s): If the device was cleared using the 510(k) pathway, identify the Predicate Device(s) K/DEN number used to claim substantial equivalence and list them here in a comma separated list exactly as they appear in the text. List the primary predicate first in the list.

K083222

Reference Device(s): Identify the Reference Device(s) K/DEN number and list them here in a comma separated list exactly as they appear in the text.

Not Found

Predetermined Change Control Plan (PCCP) - All Relevant Information for the subject device only (e.g. presence / absence, what scope was granted / cleared under the PCCP, any restrictions, etc).

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§ 862.1377 Urinary homocystine (nonquantitative) test system.

(a)
Identification. A urinary homocystine (nonquantitative) test system is a device intended to identify homocystine (an analogue of the amino acid cystine) in urine. The identification of urinary homocystine is used in the diagnosis and treatment of homocystinuria (homosystine in urine), a heritable metabolic disorder which may cause mental retardation.(b)
Classification. Class II.

0

KU279d

510(k) Summary

This summary of 510(k) safety and effectiveness information is being submitted in accordance with the requirements of SMDA 1990 and 21 CFR 807.92.

Submission correspondent:

Karen Hill Requlatory Affairs Manager Axis-Shield Diagnostics Ltd. The Technology Park Dundee DD2 1XA. Scotland, UK

Device Name: 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel®

Reagents:

Classification Name: Urinary Homocystine (Nonquantitative) Test System Trade Name: 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® Common Name: Homocysteine Enzyme Assay Governing Regulation: 862.1377 Device Classification: Class II Classification Panel: Clinical Chemistry Product Code: LPS

Legally marketed device to which equivalency is claimed:

Axis-Shield Liquid Stable (LS) 2-Part Homocysteine Reagent (K083222)

Intended Use of Device:

The 3-Reagent Homocysteine Assay for Beckman Coulter SYNCHRON® and UniCel® systems is intended for in vitro quantitative determination of total homocysteine in human serum and plasma. The device can assist in the diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria.

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Description of Device:

Bound or dimerised homocysteine (oxidised form) is reduced to free homocysteine, which then reacts with serine catalysed by cystathionine beta-synthase (CBS) to form cystathionine. Cystathionine in turn is broken down by cystathionine beta-lyase (CBL) to form homocysteine, pyruvate and ammonia. Pyruvate is then converted by lactate dehydrogenase (LDH) to lactate with nicotinamide adenine dinucleotide (NADH) as coenzyme. The rate of NADH conversion to NAD+ is directly proportional to the concentration of homocysteine (△ A340 nm).

Comparison of Technological Characteristics:

The 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® and the Axis-Shield Liquid Stable (LS) 2-Part Homocysteine Reagent are both enzymatic assays for the quantitative determination of total homocysteine in human serum and plasma. The calibrator formulations are identical and although both assays use the same cycling enzyme assay technology, the number or reagents and reagent formulations are different.

Summary of Non-Clinical Performance:

The 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® is substantially equivalent to the Axis-Shield Liquid Stable (LS) 2-Part Homocysteine Reagent in terms of precision, calibration, limit of detection and linearity on dilution as demonstrated in non-clinical performance data in this 510(k) submission.

Summary of Clinical Performance:

The 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® demonstrated substantially equivalent performance to the Axis-Shield Liquid Stable (LS) 2-Part Homocysteine Reagent as indicated by a method comparison study, in which a Passing & Bablock method comparison and a Pearson correlation analysis was conducted using 100 samples covering the full measuring range of the assay.

The 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® on the Synchron. LX Pro analyzer demonstrated substantially equivalent performance to the Axis-Shield

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Liquid Stable (LS) 2-Part Homocysteine Reagent on the Olympus AU400 analyser as indicated by a slope of 1.01 (95% Cl: 0.99 to 1.04), an intercept of 0.07 (95% Cl: -0.30 to 0.44) and a correlation coefficient (r) of 0.997 (95% CI: 0.99 to 1.00).

The 3-Reagent Homocysteine Assay for SYNCHRON® and UniCel® on the Unicel DxC analyzer demonstrated substantially equivalent performance to the Axis-Shield Liquid Stable (LS) 2-Part Homocysteine Reagent on the Olympus AU400 analyser as indicated by a slope of 0.99 (95% Cl: 0.97 to 1.02), an intercept of 0.74 (95% Cl: 0.30 to 1.11) and a correlation coefficient (r) of 0.994 (95% CI: 0.99 to 1.00).

Axis-Shield 3-Reagent Homocysteine Assay for Synchron 510(k) Premarket notification submission ADMIN 3.0 510(k) Summary Final Rev 3.0 2012-04-18

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Image /page/3/Picture/0 description: The image shows the logo for the U.S. Department of Health & Human Services. The logo consists of a circular seal with the text "DEPARTMENT OF HEALTH & HUMAN SERVICES - USA" arranged around the perimeter. Inside the circle is a stylized symbol that resembles three overlapping abstract shapes, often interpreted as human figures or flowing elements.

Public Health Service

Food and Drug Administration 10903 New Hampshire Avenue Building 66 Silver Spring, MD 20993

Axis-Shield Diagnostics Ltd. c/o Karen Hill Regulatory Manager The Technology Park Luna Place Dundee, UK, DD2 1XA, UK

MAY - 7 2012

K112790 Re:

Trade/Device Name: 3-Reagent Homocysteine Assay for Synchron® and Unicel® Regulation Number: 21 CFR § 862.1377 Regulation Name: Urinary Homocysteine (Nonquantitative) Test System Regulatory Class: Class II Product Code: LPS Dated: March 30, 2012 Received: April 3, 2012

Dear Karen Hill:

We have reviewed your Section 510(k) premarket notification of intent to market the device referenced above and have determined the device is substantially equivalent (for the indications for use stated in the enclosure) to legally marketed predicate devices marketed in interstate commerce prior to May 28, 1976, the enactment date of the Medical Device Amendments, or to devices that have been reclassified in accordance with the provisions of the Federal Food, Drug, and Cosmetic Act (Act) that do not require approval of a premarket approval application (PMA). You may, therefore, market the device, subject to the general controls provisions of the Act. The general controls provisions of the Act include requirements for annual registration, listing of devices, good manufacturing practice, labeling, and prohibitions against misbranding and adulteration.

If your device is classified (see above) into either class II (Special Controls) or class III (PMA), it may be subject to such additional controls. Existing major regulations affecting your device can be found in Title 21, Code of Federal Regulations (CFR), Parts 800 to 895. In addition, FDA may publish further announcements concerning your device in the Federal Register.

Please be advised that FDA's issuance of a substantial equivalence determination does not mean r reaso oe ac nove a determination that your device complies with other requirements of the Act that I Dr. I has made sond regulations administered by other Federal agencies. You must comply or any I detail claracts and enterests, including, but not limited to: registration and listing (21 CFR Part 807); labeling (21 CFR Parts 801 and 809); medical device reporting (reporting of medical device-607), laboling (21 CFR 803); and good manufacturing practice requirements as set forth in the quality systems (QS) regulation (21 CFR Part 820).

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If you desire specific advice for your device on our labeling regulation (21 CFR Part 801), please contact the Office of In Vitro Diagnostic Device Evaluation and Safety at (301) 796-5450. Also, please note the regulation entitled, "Misbranding by reference to premarket notification" (21 CFR Part 807.97). For questions regarding postmarket surveillance, please contact CDRH's Office of Surveillance and Biometric's (OSB's) Division of Postmarket Surveillance at (301) 796-5760. For questions regarding the reporting of adverse events under the MDR regulation (21 CFR Part 803), please go to http://www.fda.gov/Medical

Devices/Safety/ReportaProblem/default.htm for the CDRH's Office of Surveillance and Biometrics/Division of Postmarket Surveillance ...

You may obtain other general information on your responsibilities under the Act from the Division of Small Manufacturers, International and Consumer Assistance at its toll-free number (800) 638-2041 or (301) 796-5680 or at its Internet address http://www.fda.gov/MedicalDevices/ResourcesforYou/Industry/default.htm

Sincerely vours.

V.
George H. Liggins, Ph.D.

Courtney H. Lias, Ph.D. Director Division of Chemistry and Toxicology Devices Office of In Vitro Diagnostic Device Evaluation and Safety Center for Devices and Radiological Health

Enclosure

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Indication for Use

510(k) Number (if known):

KII2790 Device Name:

3-Reagent Homocysteine Assay for SYNCHRON® and Unicel®

Indication For Use:

The 3-Reagent Homocysteine Assay for Beckman Coulter SYNCHRON® and UniCel® systems is intended for in vitro quantitative determination of total homocysteine in human serum and plasma. The device can assist in the diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocysturia.

Prescription Use _ X (21 CFR Part 801 Subpart D) And/Or

Over the Counter Use (21 CFR Part 801 Subpart C)

(PLEASE DO NOT WRITE BELOW THIS LINE; CONTINUE ON ANOTHER PAGE IF NEEDED)

Concurrence of CDRH, Office of In Vitro Diagnostic Device Evaluation and Safety (OIVD)

Ruta Clima

Division Sign-Off Office of In Vitro Diagnostic Device Evaluation and Safety

510(k) K112790