K Number
K051472
Device Name
BIOPHEN LMWH CONTROL, LMWH CONTROL LOW, UFH CONTROL, HEPARIN CALIBRATOR & UFH CALIBRATOR
Manufacturer
Date Cleared
2005-12-23

(203 days)

Product Code
Regulation Number
864.5425
AI/MLSaMDIVD (In Vitro Diagnostic)TherapeuticDiagnosticis PCCP Authorized
Intended Use
Biophen Low Molecular Weight Heparin (LMWH), Low Molecular Weight Heparin Low (LMWH Low) and Unfractionated Heparin (UFH) control are set of control plasmas for the quality control of Low Molecular weight Heparin(LMWH) and Unfractionated Heparin(UFH) measurements using anti Xa colorimetric assays (Biophen Heparin 3 & 6) . These control plasmas are within the usual therapeutic range recommended for the low molecular weight heparin (LMWH) and Unfractionated Heparin (UFH). Biophen Heparin & Unfractionated heparin (UFH) Calibrators are set of calibration plasmas for the calibration of Low molecular weight heparin (LMWH) and Unfractionated heparin, using anti-Xa colorimetric assays.
Device Description
Biophen LMWH, LMWH Low and UFH Control Plasma is an in vitro diagnostic quality control intended for use with chromogenic assays to assess precision and accuracy at Heparin low and high levels (in the usual recommended therapeutic range). Biophen Heparin Calibrator is a set of calibration plasmas for Heparin (UFH and LMWH) measurements, using anti-Xa colorimetric assays (BIOPHEN HEPARIN 3 and 6). Biophen Heparin Calibrator allows calibrating the assays of Low Molecular Weight Heparin (LMWH) using chromogenic anti-Xa methods. It can be also used for calibrating the measurements of Unfractionated Heparin (UFH) when the BIOPHEN Heparin kit is used. Biophen heparin is a chromogenic anti-Xa method developed for measuring homogeneously heparin (UFH) and Low Molecular Weight Heparin (LMWH), using the same calibration curve. BIOPHEN UFH Calibrator is a set of calibration plasmas for Unfractionated Heparin (UFH) measurements, using anti-Xa colorimetric assays (BIOPHEN HEPARIN 3 and 6). BIOPHEN UFH Calibrator allows calibrating the measurements of Unfractionated Heparin (UFH) when the BIOPHEN Heparin kit is used.
More Information

Not Found

No
The document describes in vitro diagnostic control and calibration plasmas for heparin measurements using colorimetric assays. There is no mention of AI or ML technology in the intended use, device description, or performance studies.

No.
This device is an in vitro diagnostic quality control and calibration plasma for measuring heparin levels, not a therapeutic device that directly treats patients.

No

Explanation: The device is described as an "in vitro diagnostic quality control intended for use with chromogenic assays to assess precision and accuracy" and "calibration plasmas." It is used to calibrate and control other diagnostic devices (anti-Xa colorimetric assays) that measure heparin levels, rather than directly diagnosing a patient's condition.

No

The device description clearly states it is a "set of control plasmas" and "set of calibration plasmas," which are physical substances used in in vitro diagnostic testing, not software.

Yes, this device is an IVD (In Vitro Diagnostic).

The document explicitly states in the "Device Description" section:

  • "Biophen LMWH, LMWH Low and UFH Control Plasma is an in vitro diagnostic quality control..."

Furthermore, the intended use and description clearly indicate that these products are used to calibrate and control assays that measure substances (Heparin) in biological samples (plasma) outside of the body, which is the definition of an in vitro diagnostic device.

N/A

Intended Use / Indications for Use

Biophen Low Molecular Weight Heparin (LMWH), Low Molecular Weight Heparin Low (LMWH Low) and Unfractionated Heparin (UFH) control are set of control plasmas for the quality control of Low Molecular weight Heparin(LMWH) and Unfractionated Heparin(UFH) measurements using anti Xa colorimetric assays (Biophen Heparin 3 & 6) .

These control plasmas are within the usual therapeutic range recommended for the low molecular weight heparin (LMWH) and Unfractionated Heparin (UFH).

Biophen Heparin & Unfractionated heparin (UFH) Calibrators are set of calibration plasmas for quantitative determination of Low molecular weight heparin (LMWH) and Unfractionated heparin, using anti-Xa colorimetric assays.

Product codes (comma separated list FDA assigned to the subject device)

GGN, GGC, KFF

Device Description

Biophen LMWH, LMWH Low and UFH Control Plasma is an in vitro diagnostic quality control intended for use with chromogenic assays to assess precision and accuracy at Heparin low and high levels (in the usual recommended therapeutic range).

Biophen Heparin Calibrator is a set of calibration plasmas for Heparin (UFH and LMWH) measurements, using anti-Xa colorimetric assays (BIOPHEN HEPARIN 3 and 6). Biophen Heparin Calibrator allows calibrating the assays of Low Molecular Weight Heparin (LMWH) using chromogenic anti-Xa methods. It can be also used for calibrating the measurements of Unfractionated Heparin (UFH) when the BIOPHEN Heparin kit is used. Biophen heparin is a chromogenic anti-Xa method developed for measuring homogeneously heparin (UFH) and Low Molecular Weight Heparin (LMWH), using the same calibration curve.

BIOPHEN UFH Calibrator is a set of calibration plasmas for Unfractionated Heparin (UFH) measurements, using anti-Xa colorimetric assays (BIOPHEN HEPARIN 3 and 6). BIOPHEN UFH Calibrator allows calibrating the measurements of Unfractionated Heparin (UFH) when the BIOPHEN Heparin kit is used.

Mentions image processing

Not Found

Mentions AI, DNN, or ML

Not Found

Input Imaging Modality

Not Found

Anatomical Site

Not Found

Indicated Patient Age Range

Not Found

Intended User / Care Setting

Not Found

Description of the training set, sample size, data source, and annotation protocol

Not Found

Description of the test set, sample size, data source, and annotation protocol

Not Found

Summary of Performance Studies (study type, sample size, AUC, MRMC, standalone performance, key results)

A reproducibility results for the Biophen LMWH, LMWH low and UFH Control plasma is given below:

Biophen LMWH Control:
Level 3: LMWH Concentration 0.79 IU/ml, Acceptable Range 0.69 -0.89 IU/ml, N=69, SD=0.03
Level 4: LMWH Concentration 1.25 IU/ml, Acceptable Range 1.10-1.40 IU/ml, N=69, SD=0.05

Biophen LMWH Control Low:
Level I: LMWH Low Concentration 0.25 IU/ml, Acceptable Range 0.17-0.33 IU/ml, N=43, SD=0.02
Level II: LMWH Low Concentration 0.50 IU/ml, Acceptable Range 0.40-0.60 IU/ml, N=43, SD=0.03

Biophen UFH Control:
Level 1: UFH Concentration 0.21 IU/ml, Acceptable Range 0.11-0.31 IU/ml, N=30, SD=0.01
Level 2: UFH Concentration 0.51 IU/ml, Acceptable Range 0.36-0.66 IU/ml, N=30, SD=0.02

The concentration of the calibrators is determined from the multiple determinations. The following tables show good reproducibility for the LMWH calibrator and UFH calibrator.

LMWH Calibrator:
Calibrator 1: Concentration 0 IU/ml LMWH, Intra Assay N=10, Intra Assay CV=NA, Inter Assay N=50, Inter Assay SD=0.01
Calibrator 2: Concentration 0.38 IU/ml LMWH, Intra Assay N=10, Intra Assay CV=2.3, Inter Assay N=50, Inter Assay SD=0.02
Calibrator 3: Concentration 0.77 IU/ml LMWH, Intra Assay N=10, Intra Assay CV=0.5, Inter Assay N=50, Inter Assay SD=0.03
Calibrator 4: Concentration 1.14 IU/ml LMWH, Intra Assay N=10, Intra Assay CV=1.0, Inter Assay N=50, Inter Assay SD=0.05
Calibrator 5: Concentration 1.5 IU/ml LMWH, Intra Assay N=10, Intra Assay CV=0.5, Inter Assay N=50, Inter Assay SD=0.06

Unfractionated Heparin (UFH) Calibrator:
Calibrator 1: Concentration 0 IU/ml, Intra Assay N=10, Intra Assay CV=NA, Inter Assay N=28, Inter Assay SD=0
Calibrator 2: Concentration 0.36 IU/ml, Intra Assay N=10, Intra Assay CV=1.4, Inter Assay N=28, Inter Assay SD=0.02
Calibrator 3: Concentration 0.74 IU/ml, Intra Assay N=10, Intra Assay CV=1.0, Inter Assay N=28, Inter Assay SD=0.04
Calibrator 4: Concentration 1.08 IU/ml, Intra Assay N=10, Intra Assay CV=0.5, Inter Assay N=28, Inter Assay SD=0.06
Calibrator 5: Concentration 1.42 IU/ml, Intra Assay N=10, Intra Assay CV=0.5, Inter Assay N=28, Inter Assay SD=0.08

Key Metrics (Sensitivity, Specificity, PPV, NPV, etc.)

Not Found

Predicate Device(s): If the device was cleared using the 510(k) pathway, identify the Predicate Device(s) K/DEN number used to claim substantial equivalence and list them here in a comma separated list exactly as they appear in the text. List the primary predicate first in the list.

K030965, K943520, K030964, K042941

Reference Device(s): Identify the Reference Device(s) K/DEN number and list them here in a comma separated list exactly as they appear in the text.

Not Found

Predetermined Change Control Plan (PCCP) - All Relevant Information for the subject device only (e.g. presence / absence, what scope was granted / cleared under the PCCP, any restrictions, etc).

Not Found

§ 864.5425 Multipurpose system for in vitro coagulation studies.

(a)
Identification. A multipurpose system for in vitro coagulation studies is a device consisting of one automated or semiautomated instrument and its associated reagents and controls. The system is used to perform a series of coagulation studies and coagulation factor assays.(b)
Classification. Class II (special controls). A control intended for use with a multipurpose system for in vitro coagulation studies is exempt from the premarket notification procedures in subpart E of part 807 of this chapter subject to the limitations in § 864.9.

0

K 051472

Section O. Biophen LMWH and UFH Control Plasma

(Summary of Safety and Effectiveness)

Submitted by:

Hyphen BioMed 95000 Neuville sur Oise, France Phone # 01 34 40 6510 Fax# 01 34 487236

Contact Person:

Dr. Jean Amiral, President &Scientific Director jamiral@hyphen-Biomed.com

Summary prepared by:

15th April 2005

Name of the device:

Biophen LMWH Control Biophen LMWH Control Low ಹಿ Biophen UFH Control

Classification Name:

Plasma Coagulation Control

Classification: Class II

Regulation#: 864.5425, for Biophen LMWH & LMWH Low Control Plasma 864.5425 for Biophen UFH control plasma

  • Product Code: GGN for Biophen LMWH & LMWH Low Control Plasma GGN for Biophen UFH Control Plasma

Predicate Device Information:

Biophen LMWH & LMWH Low Control Plasma: Control Plasma LMW Heparin (K030965) Siophen Linker Pasma: Heparin Control Plasma: Sontrol Plasma: Biomor Plasma: Biogrich Control (Kosopori
Biophen UFH Control Plasma: Heparin Control (K943520) — Theri

Description of the Device/intended use:

Biophen LMWH, LMWH Low and UFH Control Plasma is an in vitro diagnostic quality control intended for use with chromogenic assays to assess precision and accuracy at Heparin low and high levels (in the usual recommended therapeutic range).

Statement of how the technological Characteristics of the device compare to the Predicate device:

Biophen LMWH control uses the same principle as the predicate device Control Plasma LMW Heparin and is substantially equivalent in performance, intended use and safety and effectiveness.

Hyphen Biomed 510(k) for Biophen LMWH, LMWH Low control and UFH Control Plasma

1

Biophen UFH control uses the same principle as the predicate device Heparin Control and is substantially equivalent in performance, intended use and safety and effectiveness.

Summary of Performance data:

A reproducibility results for the Biophen LMWH, LMWH low and UFH Control plasma is given below:

| Biophen
LMWH
Control | LMWH
Concentration
(IU/ml) | Acceptable
Range (IU/ml) | N | SD |
|----------------------------|----------------------------------|-----------------------------|----|------|
| Level 3 | 0.79 | 0.69 -0.89 | 69 | 0.03 |
| Level 4 | 1.25 | 1.10-1.40 | 69 | 0.05 |

| Biophen
LMWH
Control
Low | LMWH Low
Concentration
(IU/ml) | Acceptable
Range (IU/ml) | N | SD |
|-----------------------------------|--------------------------------------|-----------------------------|----|------|
| Level I | 0.25 | 0.17-0.33 | 43 | 0.02 |
| Level II | 0.50 | 0.40-0.60 | 43 | 0.03 |
| Biophen
UFH
Control | UFH
Concentration
(IU/ml) | Acceptable
Range (IU/ml) | N | SD |
| Level 1 | 0.21 | 0.11-0.31 | 30 | 0.01 |
| Level 2 | 0.51 | 0.36-0.66 | 30 | 0.02 |

2

(Summary of Safety and Effectiveness)

Submitted by:

Hyphen BioMed 95000 Neuville sur Oise, France Phone # (33) 01 34 40 6510 Fax# (33) 01 34 487236

Contact Person:

Dr. Jean Amiral, President &Scientific Director Phone number : (+33)(1)34406510 jamiral@hyphen-Biomed.com

US Agent :

Mr. Ola Andersson Phone number : (513) 770-1993 Ola@aniara.com

Summary prepared by:

3rd November 2005

Name of the device:

Biophen Heparin Calibrator Biophen UFH Calibrator

Classification Name:

Calibrator, Secondary

Classification:

Class II

Regulation#: 862.1150

Product Code: JIT

Predicate Device Information: K030964 Calibration Plasma LMW Heparin K042941 Heparin Calibrators & Controls

Device intended use:

Biophen Heparin Calibrator is a set of calibration plasmas for Heparin (UFH and LMWH) measurements, using anti-Xa colorimetric assays (BIOPHEN HEPARIN 3 and 6). Biophen Heparin Calibrator allows calibrating the assays of Low Molecular Weight Heparin (LMWH) using chromogenic anti-Xa methods. It can be also used for calibrating the measurements of Unfractionated Heparin (UFH) when the BIOPHEN Heparin kit is used. Biophen heparin is a chromogenic anti-Xa method developed for measuring homogeneously heparin (UFH) and Low Molecular Weight Heparin (LMWH), using the same calibration curve.

K051472 Response to 7/6/05 Deficiency Letter October 31, 2005

3

BIOPHEN UFH Calibrator is a set of calibration plasmas for Unfractionated Heparin (UFH) measurements, using anti-Xa colorimetric assays (BIOPHEN HEPARIN 3 and 6). BIOPHEN UFH Calibrator allows calibrating the measurements of Unfractionated Heparin (UFH) when the BIOPHEN Heparin kit is used.

Performance of the Device:

The concentration of the calibrators is determined from the multiple determinations. The following tables show good reproducibility for the LMWH calibrator and UFH calibrator.

| Calibrator | Concentration in
LMWH (IU/ml) | Intra
Assay
N | Intra
Assay
CV | Inter
Assay
N | Inter
Assay
SD |
|--------------|----------------------------------|---------------------|----------------------|---------------------|----------------------|
| Calibrator 1 | 0 | 10 | NA | 50 | 0.01 |
| Calibrator 2 | 0.38 | 10 | 2.3 | 50 | 0.02 |
| Calibrator 3 | 0.77 | 10 | 0.5 | 50 | 0.03 |
| Calibrator 4 | 1.14 | 10 | 1.0 | 50 | 0.05 |
| Calibrator 5 | 1.5 | 10 | 0.5 | 50 | 0.06 |

| Calibrator | Concentration in
Unfractionated
Heparin (IU/ml) | Intra
Assay
N | Intra
Assay
CV | Inter
Assay
N | Inter
Assay
SD |
|--------------|-------------------------------------------------------|---------------------|----------------------|---------------------|----------------------|
| Calibrator 1 | 0 | 10 | NA | 28 | 0 |
| Calibrator 2 | 0.36 | 10 | 1.4 | 28 | 0.02 |
| Calibrator 3 | 0.74 | 10 | 1.0 | 28 | 0.04 |
| Calibrator 4 | 1.08 | 10 | 0.5 | 28 | 0.06 |
| Calibrator 5 | 1.42 | 10 | 0.5 | 28 | 0.08 |

4

Image /page/4/Picture/1 description: The image shows the logo for the U.S. Department of Health & Human Services. The logo consists of a circular seal with the text "DEPARTMENT OF HEALTH & HUMAN SERVICES - USA" arranged around the perimeter. Inside the circle is a stylized image of an eagle with three wavy lines representing its body and wings.

Food and Drug Administration HYPHEN Biomed 2098 Gaither Road Rockville MD 20850 c/o Mr. Ola Anderson DEC 2 3 2005 President Aniara Corporation 6560 Gove Court Mason, Ohio 45040 K051472 Re: Trade/Device Name: Biophen Low Molecular Weight Heparin (LMWH) Control Plasma Biophen Low Molecular Weight Heparin (LMWH) Control Low Biophen Unfractionated Heparin (UFH) Control Plasma Biophen Heparin Calibrator and Biophen UFH Calibrator Regulation Number: 21 CFR § 864.5425 Regulation Name: Multipurpose system for in vitro coagulation studies Regulatory Class: II Product Code: GGN, GGC, KFF Dated: November 3, 2005

Received: November 8, 2005

Dear Ms. Anderson:

We have reviewed your Section 510(k) premarket notification of intent to market the device referenced above and have determined the device is substantially equivalent (for the indications for use stated in the enclosure) to legally marketed predicate devices marketed in interstate for workers prior to May 28, 1976, the enactment date of the Medical Device Amendments, or to devices that have been reclassified in accordance with the provisions of the Federal Food, Drug, and Cosmetic Act (Act) that do not require approval of a premarket approval application (PMA). You may, therefore, market the device, subject to the general controls provisions of the Act. The general controls provisions of the Act include requirements for annual registration, listing of devices, good manufacturing practice, labeling, and prohibitions against misbranding and adulteration.

If your device is classified (see above) into either class II (Special Controls) or class III (PMA), it If your de roo is exactional controls. Existing major regulations affecting your device can he found in Title 21, Code of Federal Regulations (CFR), Parts 800 to 895. In addition, FDA may publish further announcements concerning your device in the Federal Register.

Please be advised that FDA's issuance of a substantial equivalence determination does not mean r that FDA has made a determination that your device complies with other requirements of the Act or any Federal statutes and regulations administered by other Federal agencies. You must or any I outhal of the Act's requirements, including, but not limited to: registration and listing (21 CFR Part 807); labeling (21 CFR Parts 801 and 809); and good manufacturing practicc requirements as set forth in the quality systems (QS) regulation (21 CFR Part 820). This letter will allow you to begin marketing your device as described in your Section 510(k) premarket notification. The FDA finding of substantial equivalence of your device to a legally marketed predicate device results in a classification for your device and thus, permits your device to proceed to the market.

5

Page 2 -

If you desire specific information about the application of labeling requirements to your device, or you destro specific infortion and advertising of your device, please contact the Office of In Vitro Diagnostic Device Evaluation and Safety at (240) 276-0484. Also, please note the regulation entitled, "Misbranding by reference to premarket notification" (21CFR Part 807.97). You may obtain other general information on your responsibilities under the Act from the I va thay ovain other gefail. International and Consumer Assistance at its toll-free number (800) 638-2041 or (301) 443-6597 or at its Internet address http://www.fda.gov/cdrh/industry/support/index.html

Sincerely yours,

Robert Beckerf

Robert L. Becker, Jr., MD, P Director Division of Immunology and Hematology Office of In Vitro Diagnostic Device Evaluation and Safety Center for Devices and Radiological Health

Enclosure

6

Section D. Statement of Indication for Use Indications for Use

510(k) Number (if known): K051472

Device Name: Biophen Low Molecular Weight Heparin (LMWH) Control Plasma,

Biophen Low Molecular Weight Heparin (LMWH) Control Low,

& Biophen Unfractionated Heparin (UFH) Control Plasma

Indications for Use:

، ﻣﺪﺭ ﻣ

Biophen Low Molecular Weight Heparin (LMWH), Low Molecular Weight Heparin Low (LMWH Low) and Unfractionated Heparin (UFH) control are set of control plasmas for the quality control of Low Molecular weight Heparin(LMWH) and Unfractionated Heparin(UFH) measurements using anti Xa colorimetric assays (Biophen Heparin 3 & 6) .

These control plasmas are within the usual therapeutic range recommended for the low molecular weight heparin (LMWH) and Unfractionated Heparin (UFH).

Prescription Use (Part 21 CFR 801 Subpart D)

AND/OR

Over-The-Counter Use (21 CFR 801 Subpart C)

(PLEASE DO NOT WRITE BELOW THIS LINE-CONTINUE ON ANOTHER PAGE OF NEEDED)

Concurrence of CDRH, Office of Device Evaluation (ODE)

Josephine Rutati

Division Sign/O

Office of In Vitro Diagnostic Device Evaluation and Sufery

.?)(k)` K051472

November 10, 2005 Amendment 510(k)# K021472

2

7

Section E. Statement of Indication for Use

Indications for Use

510(k) Number (if known): ___K051472

Device Name: Biophen Heparin Calibrator &

Biophen UFH calibrator

Indications for Use:

Biophen Heparin & Unfractionated heparin (UFH) Calibrators are set of calibration Drophen Freparin of Cow molecular weight heparin (LMWH) and Unfractionated heparin, using anti-Xa colorimetric assays.

Prescription Use X (Part 21 CFR 801 Subpart D)

AND/OR

Over-The-Counter Use (21 CFR 801 Subpart C)

(PLEASE DO NOT WRITE BELOW THIS LINE-CONTINUE ON ANOTHER PAGE OF NEEDED)

Concurrence of CDRH, Office of Device Eyaluation (ODE)

Ausliss Bautista
Division Sign-Off

Office of In Vitro Diagnostic Device Evaluation and Safety

510(k) Kc51472

. ...

1

November 10, 2005 Amendment 510(k)# K051472