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The Gamma Phage assay is a lytic phage assay specific for Bacillus anthracis. The Gamma Phage assay (common name) can be used on suspect non-hemolytic, aerobic, gram-positive, "ground-glass"- appearing colonies from sheep blood agar in conjunction with other markers and testing for the identification of Bacillus anthracis. The assay is not intended for screening of blood or plasma donors. Use of this assay is limited to designated laboratory Response Network (LRN) and Department of Defense (D0D).

The gamma phage is a lytic bacteriophage which binds to specific cell-surface components of susceptible bacteria. Their DNA is injected into the bacterium. The gamma phage replicate within the bacterium and produce PlyG lysine (2), resulting in lysis of the infected cell and release of phage. The release of newly synthesized phage leads to another round of phage infection and lysis. Materials Supplied: - Bacillus anthracis Gamma Phage Suspension, 0.5 ml - . Positive Control, Bacillus anthracis Pasteur Strain Spore Suspension, 1.0 ml - Negative Control, Bacillus cereus Spore Suspension, 1.0 ml ● Materials required but not supplied: - . 5% Sheep Blood Agar plate - . Inoculating loops, 1ul and 10 ul - Aerosol resistant pipette tips - . Disinfectant Equipment required: - Pipettor, 5-50 µ1 - Incubator, 35+/- 2 °C - Biological Safety Cabinet, Class II - Refrigerator, 2-8 ℃ .