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CANTROL Serum Iron and Total Iron Binding Capacity test system provides a simple, but effective, method for quantitating the level of serum iron and total iron binding capacity in human blood serum.

In the CANTROL Serum Iron procedure Iron is released from its carner, transferrin, by an acidic reagent containing hydroxylamine and thiourea which reduces the iron to its ferrous state. A chromogen reagent, ferene, reacts with the ferrous iron to produce a colored complex, the color of which is proportional to the quantity of Iron in the test sample. Color of the reaction is measured spectrophotometrically. Total Iron Binding Capacity analysis uses the same color reaction to test serum samples which have been saturated with iron by use of an ion exchange resin in the presence of ferric ammonium citrate, prior to the iron determination. The bound iron remaining in the serum after treatment with the resin compound is the Total Iron Binding Capacity.