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The ViraSTAT® FITC-Labeled Anti-Influenza test panel is intended for the qualitative detection and confirmation of influenza A and B virus isolates from infected cell cultures through the use of the ViraSTAT® FITC-Labeled Anti-Influenza Pool and the identification and confirmation of influenza A and B by typing with separate ViraSTAT® FITC-Labeled Anti-Influenza type A or B, monoclonal antibodies, respectively. Performance characteristics have not been established for direct specimen staining.

The ViraSTAT® FITC-Labeled Anti-Influenza A and B monoclonal antibodies are fluorescently-labeled antibodies for use in culture confirmation of influenza A and B infections, respectively, in standard cell culture. The virus to be detected is grown in the appropriate cell culture system, fixed on a slide or coverslip and then the cell preparation is stained with the fluorescently-labeled monoclonal antibody. The stained sample is then viewed under a fluorescent microscope for a positive or negative identification. A positive sample is determined when cells displaying typical apple-green fluorescence are observed. Fluorescence may be present in the nucleus alone, in the nucleus and the cytoplasm, or in the cytoplasm alone. A negative sample is determined when slide wells or coverslips show no specific apple-green fluorescence in the cells and have at least 50 intact red counterstaining cells per well or 50% of the monolayer remaining on the coverslips or slides that are counterstained red.