K Number
K200788
Manufacturer
Date Cleared
2022-05-23

(788 days)

Product Code
Regulation Number
862.1345
Reference & Predicate Devices
Predicate For
AI/MLSaMDIVD (In Vitro Diagnostic)TherapeuticDiagnosticis PCCP AuthorizedThirdpartyExpeditedreview
Intended Use

The Assure Titanium Blood Glucose Monitoring System consists of the Assure Titanium Blood Glucose meter and the Assure Titanium Blood Glucose test strips. The Assure Titanium Blood Glucose Monitoring System is intended for use in the quantitative measurement of glucose in fresh capillary whole blood samples drawn from the fingertips. The system is intended for in vitro diagnostic, point of care use in endocrinology clinics and nursing facilities, for multiple patient use. This system should only be use, auto-disabling lancing devices for drawing finger stick capillary blood.

The system is not intended for the screening or diagnosis of diabetes mellitus but is indicated for use in determining dysglycemia.

The system is not intended for use in acute care or hospital settings.

The system is not intended for neonatal use.

The system is for prescription use only.

Device Description

The Assure Titanium Blood Glucose Monitoring System consists of a battery-powered meter, disposable test strips Assure Titanium Blood Glucose Test Strips, and control solutions. The Assure Titanium Blood Glucose Test Strips utilizes biosensor technology for the quantitative determination of glucose concentrations in capillary blood samples. Glucose in the blood reacts with the reagent in the test strip, and this produces a small electric current (amperometry). The strength of this current is proportional to the concentration of glucose in the blood. The meter measures this current and calculates the patient's glucose level.

AI/ML Overview

The Assure® Titanium Blood Glucose Monitoring System underwent extensive performance testing to demonstrate its safety and effectiveness.

1. Table of Acceptance Criteria and Reported Device Performance:

Test CategoryAcceptance CriteriaReported Device Performance
PrecisionWithin-run Precision: %CV ≤ 4.2%Within-run Precision: Met %CV ≤ 4.2% across all glucose levels (30-400 mg/dL) and all three test strip lots. For example, for Lot 280-2, %CV ranged from 2.1% to 3.1%. For Lot 280-4, %CV ranged from 1.9% to 2.9%. For Lot 280-5, %CV ranged from 2.3% to 2.7%.
Intermediate Precision: Not explicitly stated with a numerical criterion in the document for all levels, but the document states "Intermediate precision met the overall acceptance criteria."Intermediate Precision: All Lots Combined results showed %CV ranging from 1.4% to 2.1% across glucose levels from 30-400 mg/dL.
LinearityAll measurements to fall within ±15% at <75 mg/dL and ±12% at ≥75 mg/dL from reference. Data analyzed according to CLSI EP6-A.Linearity: All three test strip lots (Type 280-1, Type 302-1, Type 302-2) showed 100% of measurements falling within ±15% at <75 mg/dL and ±12% at ≥75 mg/dL from the YSI 2300 reference. R² values were 0.9988, 0.9993, and 0.9999 respectively, indicating a strong linear correlation.
HematocritFor glucose levels ≥ 75 mg/dL, average bias to comparator method <5% with no individual value exceeding 10%. For glucose levels <75 mg/dL, absolute bias with 95% confidence intervals justified for clinical impact.Hematocrit: For glucose levels ≥75 mg/dL, the results met the target acceptance criteria. For glucose levels <75 mg/dL, the absolute bias ranged from -1.5 to 2.2 mg/dL, which was considered clinically insignificant. Supports use across the claimed hematocrit range of 10-70%.
InterferenceFor each concentration of potential interferent, the average percent bias and 95% confidence interval of test sample to untreated control sample must be within ±10% at each glucose level.Interference: Not explicitly stated for all 47 substances for all glucose levels, but the study notes that it was evaluated at clinically relevant concentrations and reported the "Highest concentration with no interference" for each substance. For example, Acetaminophen showed no interference at 20 mg/dL, Ascorbic acid at 4 mg/dL. The document implies these results met the acceptance criteria by stating "The results were compared to the following target acceptance criteria: for each concentration of potential interferent the average percent bias and 95% confidence interval of test sample to untreated control sample must be within ±10% at each glucose level." However, it explicitly states, "Patients with high doses of Vitamin C intake (ascorbic acid; blood levels higher than . 4 mg/dL) may yield inaccurate results. Patients undergoing oxygen therapy may yield inaccurate results."
Cleaning & Disinfection Viral Elimination EffectivenessNot explicitly stated in the provided text, but implies effective viral elimination.Cleaning & Disinfection Viral Elimination Effectiveness: PDI Super Sanicloth Wipes were proven effective in eliminating duck hepatitis B virus (a surrogate for human hepatitis B virus) from all tested surfaces of the device.
Cleaning & Disinfection Robustness/DurabilityThe system should not be adversely affected by recommended cleaning and disinfection cycles over a three-year use life (10,950 cycles).Cleaning & Disinfection Robustness/Durability: After 10,950 cleaning and disinfection cycles using PDI Super Sanicloth Wipes, the device maintained venous blood performance within acceptance criteria and passed physical and performance inspections.
Intermittent SamplingThe system should provide accurate glucose measurements or an error when the sample is intermittently applied.Intermittent Sampling: The results demonstrated that the system "provides accurate glucose measurements or an error when the sample is intermittently applied."
Environmental/System Operating ConditionsNot explicitly stated, but implies performance within and outside claimed operating range.Environmental/System Operating Conditions: A study was performed to assess performance under various temperature and humidity conditions, covering and exceeding the claimed operating range. The studies were designed to represent actual use conditions.
AltitudePerformance should be unaffected by altitudes up to the claimed range.Altitude: Performance was demonstrated to be unaffected by altitudes up to and equal to 10,000 feet.
Error Codes for Samples Outside Measuring RangeAll meters to display "Lo" at glucose levels <10 mg/dL, and all meters to display "HI" at glucose levels >600 mg/dL.Error Codes for Samples Outside Measuring Range: All results met the acceptance criteria, displaying "Lo" for <10 mg/dL and "HI" for >600 mg/dL.
Error Codes and FlaggingFor each error code/flag, the appropriate conditions must trigger it, and conditions that should not trigger it must not.Error Codes and Flagging: For each error code/flag listed (E-1 to E-10, E6, E5, E0, Control Solution Flag, Temperature Flag), the appropriate conditions triggered the error, and non-triggering conditions did not, verifying proper functionality.
Short Sample DetectionThe system should either produce an error or an accurate result when a short sample is applied.Short Sample Detection: The system demonstrated it will either produce an error or an accurate result when short sampled (using sample volumes from 0.1 to 10 uL).
Sample PerturbationThe system should either provide an error or an accurate result when the sample is perturbed.Sample Perturbation: The system demonstrated it would either provide an error or an accurate result when the sample was perturbed (by flicking the test strip, flipping the meter, or wicking the sample away).
Testing with Used Test StripAll used test strips shall produce the E1 error upon re-insertion, and no glucose measurement results should be provided.Testing with Used Test Strip: All test results met the acceptance criteria, producing the E1 error upon re-insertion of used test strips.
Clinical Accuracy (Method Comparison)Nursing/Skilled Nursing Facility: <75 mg/dL: 100% within ±15 mg/dL. ≥75 mg/dL: 100% within ±20%. Endocrinology Clinic: <75 mg/dL: 100% within ±15 mg/dL. ≥75 mg/dL: 100% within ±20%.Nursing/Skilled Nursing Facility: <75 mg/dL: 100% (1/1) within ±5 mg/dL, ±10 mg/dL, ±12 mg/dL, ±15 mg/dL. ≥75 mg/dL: 100% (129/129) within ±20%. Endocrinology Clinic: <75 mg/dL: 100% (26/26) within ±12 mg/dL and ±15 mg/dL (96.2% within ±10 mg/dL). ≥75 mg/dL: 100% (240/240) within ±20%.
Accuracy at Extremes<80 mg/dL: 100% within ±15 mg/dL. >300 mg/dL: 100% within ±20%.Accuracy at Extremes: <80 mg/dL: 100% (50/50) within ±12 mg/dL and ±15 mg/dL (98% within ±10 mg/dL, 86% within ±5 mg/dL). >300 mg/dL: 100% (50/50) within ±12%, ±15%, and ±20% (92% within ±10%, 64% within ±5%).
UsabilityPositive feedback on ease of use and understanding of the system and manuals.Usability: 100% of POC operators provided positive feedback (Very Easy, or OK) on the ease of use of the blood glucose meter system and the user manual/quick reference guide.

2. Sample Size Used for the Test Set and the Data Provenance:

  • Precision (Within-run): 1500 tests (500 results on each of 3 lots)

  • Precision (Intermediate): 1500 tests (50 tests per lot per day over 10 days for 3 lots)

  • Linearity: 165 results (5 replicates per level for 11 glucose concentrations, across 3 test strip lots)

  • Clinical Accuracy (Method Comparison): Capillary blood samples from 396 patients were measured.

    • Site #1 (Nursing/Skilled Nursing Facility): 130 patients
    • Site #2 (Endocrinology Clinic, California): 165 patients
    • Site #3 (Endocrinology Clinic, Georgia): 101 patients
  • Accuracy at Extremes: 100 samples (50 samples <80 mg/dL, 50 samples >300 mg/dL).

    The provenance for precision, linearity, hematocrit, and interference studies appears to be laboratory-controlled (venous whole blood spiked or glycolyzed). The clinical study data provenance is prospective, collected in the United States (Massachusetts, California, Georgia) at one nursing/skilled nursing facility and two endocrinology clinics.

3. Number of Experts Used to Establish the Ground Truth for the Test Set and the Qualifications of Those Experts:

The document mentions that the YSI Model 2300 Glucose Analyzer was used as the reference standard (comparator method) for establishing ground truth in the linearity, clinical accuracy, and accuracy at extremes studies. The YSI 2300 is a laboratory instrument, not a human expert. Therefore, no human experts were used to establish the ground truth for these quantitative measurements.

For the usability study, "POC operators" were involved in evaluating the system, but they weren't establishing a "ground truth" for glucose levels. Their qualifications are not specified beyond being "Point-of-Care operators."

4. Adjudication Method for the Test Set:

Not applicable, as the ground truth for glucose measurements was established by a laboratory reference instrument (YSI 2300), not through expert consensus requiring adjudication.

5. If a Multi-Reader Multi-Case (MRMC) Comparative Effectiveness Study Was Done:

No, an MRMC comparative effectiveness study was not done. This device is a Blood Glucose Monitoring System, and its performance is evaluated by comparing its readings directly to a laboratory reference standard (YSI 2300), not by assessing how human readers improve with AI assistance.

6. If a Standalone (i.e., algorithm only without human-in-the-loop performance) Was Done:

Yes, the core performance studies (precision, linearity, hematocrit, interference, environmental, altitude, error codes, short sample detection, sample perturbation, used test strip detection) evaluate the standalone performance of the Assure® Titanium Blood Glucose Monitoring System (meter and test strips) by comparing its output directly to a reference method or evaluating built-in error detection mechanisms.

The clinical accuracy study also compares the device's measurements (obtained by POC operators, but the measurement itself is algorithmic) against the YSI 2300 reference, effectively demonstrating its standalone accuracy in a clinical setting.

7. The Type of Ground Truth Used:

The primary ground truth used for quantitative glucose measurements was reference laboratory method data, specifically from the YSI Model 2300 Glucose Analyzer.

8. The Sample Size for the Training Set:

The document does not specify a separate "training set" or its size. This is typical for a medical device cleared via a 510(k) pathway, especially for a blood glucose monitoring system, where development often involves calibration and verification/validation against reference methods rather than supervised machine learning model training.

9. How the Ground Truth for the Training Set Was Established:

Not applicable, as a separate training set and its ground truth establishment are not discussed in the provided submission. The device's underlying principles (electrochemical biosensor) are well-established, and its "training" or calibration would typically be an inherent part of its manufacturing process, verified through performance testing against reference standards as described.

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May 23, 2022

Arkray, Inc. Dhwani Thakkar Regulatory Affairs Project Manager 5182 West 76th Street Minneapolis, MN 55439

Re: K200788

Trade/Device Name: Assure® Titanium Blood Glucose Monitoring System Regulation Number: 21 CFR 862.1345 Regulation Name: Glucose Test System Regulatory Class: Class II Product Code: PZI Dated: June 14, 2021 Received: June 15, 2021

Dear Dhwani Thakkar:

We have reviewed your Section 510(k) premarket notification of intent to market the device referenced above and have determined the device is substantially equivalent (for the indications for use stated in the enclosure) to legally marketed predicate devices marketed in interstate commerce prior to May 28, 1976, the enactment date of the Medical Device Amendments, or to devices that have been reclassified in accordance with the provisions of the Federal Food, Drug, and Cosmetic Act (Act) that do not require approval of a premarket approval application (PMA). You may, therefore, market the device, subject to the general controls provisions of the Act. Although this letter refers to your product as a device, please be aware that some cleared products may instead be combination products. The 510(k) Premarket Notification Database located at https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfpmn/pmn.cfm identifies combination product submissions. The general controls provisions of the Act include requirements for annual registration, listing of devices, good manufacturing practice, labeling, and prohibitions against misbranding and adulteration. Please note: CDRH does not evaluate information related to contract liability warranties. We remind you, however, that device labeling must be truthful and not misleading.

If your device is classified (see above) into either class II (Special Controls) or class III (PMA), it may be subject to additional controls. Existing major regulations affecting your device can be found in the Code of Federal Regulations, Title 21, Parts 800 to 898. In addition, FDA may publish further announcements concerning your device in the Federal Register.

Please be advised that FDA's issuance of a substantial equivalence determination does not mean that FDA has made a determination that your device complies with other requirements of the Act or any Federal statutes and regulations administered by other Federal agencies. You must comply with all the Act's

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requirements, including, but not limited to: registration and listing (21 CFR Part 807); labeling (21 CFR Part 801 and Part 809); medical device reporting of medical device-related adverse events) (21 CFR 803) for devices or postmarketing safety reporting (21 CFR 4, Subpart B) for combination products (see https://www.fda.gov/combination-products/guidance-regulatory-information/postmarketing-safety-reportingcombination-products); good manufacturing practice requirements as set forth in the quality systems (OS) regulation (21 CFR Part 820) for devices or current good manufacturing practices (21 CFR 4, Subpart A) for combination products; and, if applicable, the electronic product radiation control provisions (Sections 531-542 of the Act); 21 CFR 1000-1050.

Also, please note the regulation entitled, "Misbranding by reference to premarket notification" (21 CFR Part 807.97). For questions regarding the reporting of adverse events under the MDR regulation (21 CFR Part 803), please go to https://www.fda.gov/medical-device-safety/medical-device-reportingmdr-how-report-medical-device-problems.

For comprehensive regulatory information about mediation-emitting products, including information about labeling regulations, please see Device Advice (https://www.fda.gov/medicaldevices/device-advice-comprehensive-regulatory-assistance) and CDRH Learn (https://www.fda.gov/training-and-continuing-education/cdrh-learn). Additionally, you may contact the Division of Industry and Consumer Education (DICE) to ask a question about a specific regulatory topic. See the DICE website (https://www.fda.gov/medical-device-advice-comprehensive-regulatoryassistance/contact-us-division-industry-and-consumer-education-dice) for more information or contact DICE by email (DICE(@fda.hhs.gov) or phone (1-800-638-2041 or 301-796-7100).

Sincerely,

Kellie Kelm, Ph.D. Director Division of Chemistry and Toxicology Devices OHT7: Office of In Vitro Diagnostics Office of Product Evaluation and Quality Center for Devices and Radiological Health

Enclosure

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Indications for Use

510(k) Number (if known) K200788

Device Name

Assure® Titanium Blood Glucose Monitoring System

Indications for Use (Describe)

· The Assure Titanium Blood Glucose Monitoring System consists of the Assure Titanium Blood Glucose meter and the Assure Titanium Blood Glucose test strips. The Assure Titanium Blood Glucose Monitoring System is intended for use in the quantitative measurement of glucose in fresh capillary whole blood samples drawn from the fingertips. The system is intended for in vitro diagnostic, point of care use in endocrinology clinics and nursing facilities, for multiple patient use. This system should only be use, auto-disabling lancing devices for drawing finger stick capillary blood.

• The system is not intended for the screening or diagnosis of diabetes mellitus but is indicated for use in determining dysglycemia.

  • · The system is not intended for use in acute care or hospital settings.
  • · The system is not intended for neonatal use.
  • · The system is for prescription use only.

Type of Use (Select one or both, as applicable)X Prescription Use (Part 21 CFR 801 Subpart D)

| | Over-The-Counter Use (21 CFR 801 Subpart C)

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1. Administrative Information

Applicant's Nameand AddressYoshiharu UehataARKRAY, Inc.Yousuien-nai, 59 Gansuin-choKamigyo-ku, Kyoto 602-0008 JapanEstablishment Registration # 3003422726
Primary ContactDhwani ThakkarRegulatory Affairs Project ManagerThakkarD@arkrayusa.com
Secondary ContactDaya Ranamukhaarachchi, PhDV.P. Regulatory and Scientific AffairsRanamukD@arkrayusa.com
Phone408-234-0804
Fax1-855-924-2725
EmailRanamukD@arkrayusa.com
Date PreparedJune 14, 2021

2. Device Information

Device

Trade NameAssure® Titanium Blood Glucose Monitoring System
510(k) NumberK200788
Classification NamePrescription Use Blood Glucose Meter for Near-PatientTesting
Common NameGlucose Test System
Product CodePZI
Classification Panel75 – Clinical Chemistry
DeviceClassification21 CFR § 862.1345

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3. Predicate Device Information

Predicate Device NamePredicate Device510(k) Number
StatStrip Glucose Hospital Meter SystemK132121

4. Device Description

The Assure Titanium Blood Glucose Monitoring System consists of a battery-powered meter, disposable test strips Assure Titanium Blood Glucose Test Strips, and control solutions. The Assure Titanium Blood Glucose Test Strips utilizes biosensor technology for the quantitative determination of glucose concentrations in capillary blood samples. Glucose in the blood reacts with the reagent in the test strip, and this produces a small electric current (amperometry). The strength of this current is proportional to the concentration of glucose in the blood. The meter measures this current and calculates the patient's glucose level.

Test principle:

The sample (whole blood) is drawn by capillary action at the tip of the test strip. As depicted in the chemical reactions listed below in Figure 1, glucose in the sample reacts with glucose oxidase (GOD) and Hexaammineruthenium (III) chloride in the test strip. This produces Hexaammineruthenium (II) chloride. Hexaammineruthenium (II) chloride is produced in proportion to the glucose concentration of the blood sample. Oxidation of the Hexaammineruthenium (II) chloride produces an electric current. The meter converts the current to the glucose concentration and displays it as the test result.

B-D-glucose + Hexaammineruthenium (II) chloride -> D-Glucono-δLactone + Hexaammineruthenium (II) chloride Hexaammineruthenium (II) chloride Electrical Charge Hexaammineruthenium (III) chloride + e-

Figure 1: Principle of Action for Assure Titanium Blood Glucose Monitoring System

A similar test principle is used in StatStrip Glucose Hospital Meter System previously cleared under K132121.

5. Indications for Use

The Assure Titanium Blood Glucose Monitoring System consists of the Assure Titanium Blood Glucose meter and the Assure Titanium Blood Glucose test

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strips. The Assure Titanium Blood Glucose Monitoring System is intended for use in the quantitative measurement of glucose in fresh capillary whole blood samples drawn from the fingertips. The system is intended for in vitro diagnostic, point of care use in endocrinology clinics and nursing or skilled nursing facilities, for multiple patient use. This system should only be used with single-use, auto-disabling lancing devices for drawing finger stick capillary blood.

The system is not intended for the screening or diagnosis of diabetes mellitus but is indicated for use in determining dysglycemia.

The system is not intended for use in acute care or hospital settings.

The system is not intended for neonatal use.

The system is for prescription use only.

6. Substantial Equivalence Information

The Assure Titanium Blood Glucose Monitoring Device uses similar intended use as the predicate, StatStrip Glucose Hospital Meter System. Also, test principle and technology are similar for both devices. Table 1 below provides a comparison between the Assure Titanium Blood Glucose Monitoring System and its predicate device.

As described in the performance testing summary below, the verification and validation (bench and clinical) testing successfully demonstrated substantial equivalence for the Assure Titanium Blood Glucose Monitoring System to the predicate device as required per 21 CFR § 807.92(b)(3).

Table 1: Similarities and Differences Table
COMPONENT/CHARACTERISTICPROPOSEDPREDICATE
510(k) NumberK200788K132121
Device/MeasuringSystemAssure® TitaniumBlood GlucoseMonitoring SystemStatStrip GlucoseHospital MeterSystem
COMPONENT/CHARACTERISTICPROPOSEDPREDICATE
Intended Use andIndications for UseThe Assure TitaniumBlood GlucoseMonitoring Systemconsists of the AssureTitanium BloodGlucose meter and theAssure TitaniumBlood Glucose teststrips. The AssureTitanium BloodGlucose MonitoringSystem is intended foruse in the quantitativeFor the quantitativedetermination ofglucose in capillaryfinger stick, venouswhole blood, arterialwhole blood, neonatearterial whole bloodand neonate heel stickspecimens. Also forthe quantitativedetermination ofglucose in venouswhole blood, arterialwhole blood,neonatal heel stick,and neonatal arterialwhole bloodthroughout allhospital and allprofessionalhealthcare settings.
intended for neonataluse.The system is forprescription use only.
Population limitationNot intended forneonatal use, acutecare or hospitalsettings, nor for usewith patientsreceiving intensivemedicalintervention/therapy.Not intended forpatients receivingintensive medicalintervention/therapy.This system isintended for use withneonatal arterial wholeblood but has not beenvalidated for neonatalvenous blood.
Sample TypeFresh capillary wholeblood*Whole Blood:Capillary, Venous,Arterial, and Neonatearterial whole blood
Test Strip EjectorYesNo
Controls3 levels of AssureControl- ControlSolutions3 levels of NovaStatStrip ControlSolutions
MaximumAltitude10,000 ft (3,048meters)15,000 ft (4500meters)
Sample Volume0.5 µL1.2 µL
Weight4.1 oz with batteries9.6 oz
Dimensions4.7 x 2.4 x 1.2 inch6.0 x 3.25 x 1.8 inch
Battery/PowersourceTwo 1.5V alkalineAAA batteries3.7V Li Polymerbattery(Rechargeable/Replaceable)
OperatingTemperature range46-104°F (8-40°C)59-104 °F (15-40°C)
Hematocrit Range10-70%20-65%
Data Storage1,000 test results1,000 Patient Tests200 QC Tests4000 Operators
Measuring Time7 seconds6 seconds
ElectricalComplianceConforms toANSI/AAMI IEC60601-1-2:2014Conforms to:IEC 61010-1:2001 andIEC 61010-2-101:2002
Measuring Range10-600 mg/dL10-600 mg/dL
Relative Humidity10-90% (no condensation)10-90% (no condensation)
EnzymeGlucose oxidase(Aspergillus niger sourced)Glucose Oxidase(Aspergillus sourced)
Test PrincipleElectro-chemicalbiosensor(Amperometric)Electro-chemicalbiosensor(Amperometric)
CalibrationAutomatic codedcalibrationAutomatic, nocalibration code
Wi-Fi networkconnectivityNoNo

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  • Although whole blood samples are used for measurement, displayed results are equivalent to plasma glucose levels.

7. Summary of Performance Testing

Performance testing was conducted on the proposed Assure Titanium Blood Glucose Monitoring System in accordance with the FDA guidance Blood Glucose Monitoring Test Systems for Prescription Point-of-Care Use, 2016 and 2020 using final product. Performance testing demonstrated that the device meets the performance requirements for its intended use.

PRECISION

Within-run and intermediate precision for the Assure Titanium Blood Glucose Monitoring System were evaluated to assess imprecision of the system across the glucose measuring range and under normal use conditions.

Briefly, within-run precision was evaluated using venous whole blood spiked with high concentration glucose solution or allowed to glycolyze to 5 glucose concentrations. These whole blood samples were tested on at least 10 vials of 3 test strip lots using at least 10 Assure Titanium Blood Glucose Meters. Test strips were taken from the same bottle for each meter. This resulted in 500 results on each lot, for a total of 1500 tests.

Results for the within-run precision testing are shown in Table 2, mean results for each glucose level are presented for each lot with associated standard deviation (SD), percent coefficient of variation (%CV) and the 95% confidence intervals.

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Lot #Glucose Level(mg/dL)Mean (mg/dL)SD95% CI%CV
280-230-5045.71.21.0 to 1.42.6%
51-11096.32.01.6 to 2.42.1%
111-150140.23.12.5 to 3.62.2%
151-250203.76.25 to 7.53.1%
251-400324.310.18.1 to 12.13.1%
280-430-5044.41.31.0 to 1.52.9%
51-11095.72.01.6 to 2.42.1%
111-150139.62.82.2 to 3.32.0%
151-250204.93.83.1 to 4.61.9%
251-400324.27.56.1 to 92.3%
280-530-5042.61.00.8 to 1.22.4%
51-11092.82.31.8 to 2.72.4%
111-150137.83.22.6 to 3.92.3%
151-250202.74.93.9 to 5.82.4%
251-400324.18.77 to 10.42.7%

Table 2: Within-Run Precision Results for Assure Titanium Blood Glucose Monitoring System

The data in Table 2 demonstrate the within-run precision met the overall acceptance criteria for %CV ≤ 4.2% for the Assure Titanium Blood Glucose Monitoring System.

Intermediate precision was evaluated using control solution adjusted to the same 5 glucose concentrations. Multiple operators tested the control solution over 10 days using at least 10 bottles of 3 test strip lots and 10 Assure Titanium meters. Test strips were taken from the same bottle for each meter. This resulted in 50 tests per lot per day for a total of 1500 tests.

Results for intermediate precision testing for all lots are shown in Table 3. Mean results for each glucose level are presented with associated standard deviation (SD), percent coefficient of variation (%CV) and the 95% confidence intervals.

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Glucose Level(mg/dL)Mean (mg/dL)SD95% CI%CV
All LotsCombined30-5042.40.80.6 to 0.91.9
51-11087.01.41.1 to 1.71.6
111-150129.31.81.4 to 2.11.4
151-250254.73.93.2 to 4.71.5
251-400360.67.56.0 to 9.02.1

Table 3: Intermediate Precision Results for Assure Titanium

The data in Table 3 show that Intermediate precision met the overall acceptance criteria.

LINEARITY

Linearity for the Assure Titanium Blood Glucose Monitoring System was evaluated using glucose concentrations across the claimed glucose measuring range and data was analyzed according to CLSI EP6-A: Evaluation of the Linearity of Quantitative Measurement: A Statistical Approach; An Approved Guideline.

Briefly, linearity was evaluated using venous whole blood spiked with high concentration glucose solution or allowed to glycolyze to eleven evenly spaced glucose concentrations. The target glucose concentrations were verified by the YSI 2300 reference analyzer. These whole blood samples were tested on 3 test strip lots using 5 Assure Titanium Blood Glucose Meters. This resulted in 5 replicates per level per lot tested, for a total of 165 results.

Data analysis included linear regression with regression equation and coefficient of determination (R2) presented for each lot tested and presented in Table 4.

LotLinear Regression EquationR2
Type 280-1y = 1.0079x + 3.94850.9988
Type 302-1y = 1.0063x + 3.37270.9993
Type 302-2y = 0.9902x + 2.18390.9999

Table 4: Linear Regression Equation for All Assure Titanium Blood Glucose Monitoring System Lots

Data analysis showed that linearity testing met the acceptance criteria in that all measurements fell within the ranges shown in Table 5, demonstrating linearity of the Assure Titanium Blood Glucose Monitoring System across the claimed measuring range of 10-600 mg/dL.

Table 5: Assure Titanium Blood Glucose Monitoring System Comparison to YSI 2300 Reference

LotWithin ±15% at <75 mg/dL and±12% at ≥75 mg/dL from reference
Type 280-1100%
Type 302-1100%
Type 302-2100%

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HEMATOCRIT

The effect of hematocrit on the performance of the Assure Titanium Blood Glucose Monitoring System was evaluated to establish that it can be safely used across the claimed hematocrit range. Briefly, percent hematocrit effect on Assure Titanium Blood Glucose Monitoring System results was evaluated using venous whole blood adjusted to five glucose levels and thirteen hematocrit levels spaced 5% apart spanning the claimed hematocrit range.

The target acceptance criteria are as follows: For glucose levels ≥ 75 mg/dL, the average bias to comparator method should be <5% with no individual value exceeding 10%. For glucose levels <75 mg/dL, the absolute bias should be reported with 95% confidence intervals justified for clinical impact.

The results for glucose levels ≥75 mg/dL met the target acceptance criteria. In addition, the absolute bias for results for glucose levels <75 mg/dL ranged from -1.5 to 2.2 mg/dL, an error so small that this error would not impact patient safety. The results of the Hematocrit Evaluation study support the safe use of the Assure Titanium Blood Glucose Monitoring System across the claimed hematocrit range of 10- 70% in the intended use population.

INTERFERENCE

The effect of potentially interfering endogenous and exogenous substances was evaluated on the Assure Titanium Blood Glucose Monitoring System. The study was designed using whole blood samples spiked or allowed to glycolyze to 3 target ranges. Eachinterferent was tested at clinically relevant concentrations. For each concentration of potential interferent, average percent bias to untreated control samples and 95% confidence intervals were calculated. If interference was observed additional testing was performed to determine the concentration at which the interference starts to occur. Table 6 lists potentially interfering substances and the maximum concentrations tested and the highest tested concentration at which no interference was observed.

Potential InterferingSubstanceHighest concentration with nointerferenceMaximum TestConcentration
1Acetaminophen20 mg/dL20 mg/dL
2Ascorbic acid4 mg/dL6 mg/dL
3Conjugated Bilirubin50 mg/dL50 mg/dL
4Unconjugated Bilirubin40 mg/dL40 mg/dL
5Cholesterol500 mg/dL500 mg/dL
6Creatinine15 mg/dL15 mg/dL
7Dopamine20 mg/dL20 mg/dL
8EDTA180 mg/dL200 mg/dL
9Galactose60 mg/dL60 mg/dL
10Gentisic acid700 mg/dL1,000 mg/dL
11Reduced Glutathione92 mg/dL92 mg/dL
12Hemoglobin20,000 mg/dL20,000 mg/dL
13Heparin500 IU/dL500 IU/dL
14Ibuprofen50 mg/dL50 mg/dL
15Icodextrin1094.4 mg/dL1094.4 mg/dL
16L-Dopa0.75mg/dL0.75 mg/dL
17Maltose5,000 mg/dL10,000 mg/dL
18Methyl-L-dopa1000 mg/dL1000 mg/dL
19Salicylic acid60 mg/dL60 mg/dL
20Sodium414 mg/dL414 mg/dL
21Tolbutamide100 mg/dL100 mg/dL
22Tolazamide40 mg/dL40 mg/dL
23Triglycerides1,500 mg/dL1,500 mg/dL
24Uric acid24 mg/dL24 mg/dL
25Xylose300 mg/dL600 mg/dL
26Xylitol0.09 mg/dL0.09 mg/dL
27Mannitol1,800 mg/dL1,800 mg/dL
28Fenofibric Acid1.8 mg/dL1.8 mg/dL
29Canagliflozin$15\times10^{-4}$ mg/dL$15\times10^{-4}$ mg/dL
30Amlodipine Besylate$18\times10^{-4}$ mg/dL$18\times10^{-4}$ mg/dL
31Atorvastatin Calcium$84\times10^{-4}$ mg/dL$84\times10^{-4}$ mg/dL
32Cilostazol$21\times10^{-2}$ mg/dL$21\times10^{-2}$ mg/dL
33Prasugrel$105\times10^{-3}$ mg/dL$105\times10^{-3}$ mg/dL
34Nortriptyline HCl$45 \times 10^{-6}$ mg/dL$45 \times 10^{-6}$ mg/dL
35Budesonide$36\times10^{-5}$ mg/dL$36\times10^{-5}$ mg/dL
36Dextromethorphan$9\times10^{-4}$ mg/dL$9\times10^{-4}$ mg/dL
37Oxcarbazepine$258\times10^{-2}$ mg/dL$258\times10^{-2}$ mg/dL
38Trihexyphenidyl HCL$15\times10^{-3}$ mg/dL$15\times10^{-3}$ mg/dL
39Fluphenazine Decanoate$81\times10^{-5}$ mg/dL$81\times10^{-5}$ mg/dL
40Levoflaxicin$183\times10^{-5}$ mg/dL$183\times10^{-5}$ mg/dL
41Glimiperide$576\times10^{-4}$ mg/dL$576\times10^{-4}$ mg/dL
42Benazeprilat297 nmol/dL297 nmol/dL
43Saxagliptin$72\times10^{-4}$ mg/dL$72\times10^{-4}$ mg/dL
44Morphine28.3 nmol/dL84.9 nmol/dL
45Ursodiol$7,836\times10^{-4}$ mg/dL$9,795\times10^{-4}$ mg/dL
46Silodosin$1848\times10^{-5}$ mg/dL$1848\times10^{-5}$ mg/dL
47Letrozole31.2 nmol/dL31.2 nmol/dL

Table 6: Maximum Concentrations Tested

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The results were compared to the following target acceptance criteria: for each concentration of potential interferent the average percent bias and 95% confidence interval of test sample to untreated control sample must be within ±10% at each glucose level.

Interfering Substances

  • Patients with high doses of Vitamin C intake (ascorbic acid; blood levels higher than . 4 mg/dL) may yield inaccurate results.
  • Patients undergoing oxygen therapy may yield inaccurate results. ●

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CLEANING AND DISINFECTION VIRAL ELIMINATION EFFECTIVENESS

The cleaning and disinfection viral elimination effectiveness study for the Assure Titanium Blood Glucose Monitoring System was conducted by Microbac Laboratories (Sterling, VA).

The elimination of duck hepatitis B virus, as a surrogate for human hepatitis B virus, using the disinfection product, PDI Super Sanicloth (EPA# 9480-4), has been evaluated with the constituent surface materials of the Assure Titanium Blood Glucose Monitoring System.

The PDI Super Sanicloth Wipes were proven to be effective in eliminating the duck hepatitis B virus from all tested surfaces.

CLEANING AND DISINFECTION ROBUSTNESS/DURABILITY

Cleaning and disinfection durability testing were performed to demonstrate that the Assure Titanium Blood Glucose Monitoring System can withstand multiple cleaning and disinfection cycles to validate a three-year use life or "warranty" for the Assure Titanium Blood Glucose Monitoring System. The number of cleaning and disinfection cvcles performed for this testing was based ten cleaning and disinfection cycles per day for three years for a total of 10.950 cycles (10 cycles/day * 365 days/year * 3 years = 10,950).

To demonstrate that the Assure Titanium Blood Glucose Monitoring System is not adversely affected by the recommended cleaning and disinfection cvcles applied over a three-vear use life. five meters were cleaned and disinfected 10,950 times with PDI Super Sanicloth Wipes (EPA# 9480-4) and examined to detect any performance issues and signs of physical deterioration.

A physical inspection and performance testing of each of the five meters was performed at the beginning of the study, midway through the study (approximately 5,480 cleaning anddisinfection cvcles), and after the 10.950 cleaning and disinfection cycles were complete. The five meters were inspected at the three time points to determine if there were signs of physical and/or performance deterioration.

The venous blood performance met the acceptance criteria at each time point. All external material passed physical and performance inspection after cleaning and disinfecting 10.950 times with PDI Super Sanicloth Wipes.

INTERMITTENT SAMPLING

Intermittent sampling occurs when a short sample is applied to a test strip, a glucose measurement begins, and more sample is applied to the test strip before the glucose measurement is complete. Briefly, intermittent sampling was evaluated using venous whole blood spiked with high concentration glucose solution or allowed to glycolyze to three concentrations: 50-65 mg/dL, 100-120 mg/dL, 200-250 mg/dL. The glucose levels were verified by the YSI 2300 reference analyzer.

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The results demonstrated that the Assure Titanium Blood Glucose Monitoring System provides accurate glucose measurements or an error when the sample is intermittently applied to an Assure Titanium Blood Glucose Test Strip.

ENVIRONMENTAL/SYSTEM OPERATING CONDITIONS

A study to assess the performance of the Assure Titanium Blood Glucose Monitoring System when used under various operating temperature and humidity conditions was performed. These studies were designed to represent actual use conditions experienced by Blood Glucose Monitoring The tested temperature and humidity ranges covered the system operating System users. conditions and also included ranges outside the claimed operating range. The testing incorporated eight extreme temperature and humidity combinations (high temperature/low humidity; high temperature/high humidity; low temperature/low humidity; low temperature/high humidity) within and outside the claimed operating range.

ALTITUDE

ARKRAY evaluated the effect of altitude on the performance of the Assure Titanium Blood Glucose Monitoring System. Three lots of Assure Titanium test strips were tested with venous blood samples at three glucose levels at two altitude conditions. Sea level (203 feet) and high altitude (10.000 feet) were tested using a custom-made pressure chamber to create the effects of altitude.

The results demonstrated that the Assure Titanium Blood Glucose Monitoring System performance is unaffected by altitudes up to and equal to an elevation of 10,000 feet.

ERROR CODES FOR SAMPLES OUTSIDE THE MEASURING RANGE

ARKRAY performed analyses to demonstrate that the Assure Titanium Blood Glucose Monitoring System provides the appropriate error codes when measured glucose concentrations are outside of the Blood Glucose Monitoring System's claimed measuring range. Three lots of Assure Titanium test strips were tested with venous blood samples at two glucose levels (<10 and >600 mg/dL). The acceptance criteria were as follows: All meters to display "Lo" at glucose levels <10 mg/dL, all meters to display "HI" at glucose levels >600 mg/dL.

All results met the acceptance criteria demonstrating that the Assure Titanium Blood Glucose Monitoring System will supply the user with the appropriate error codes when measuring blood glucose concentrations outside of the claimed measuring range.

ERROR CODES AND FLAGGING

ARKRAY performed evaluations to demonstrate that the Assure Titanium Blood Glucose Monitoring System provides error codes and flags according to the design specification. Please see Table 7 below for details on the error codes/flags evaluated.

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Table 7: Assure Titanium Blood Glucose Monitoring System Error Codes and Flags
Error CodeDescription
E-1Test strip was previously used. Retest with a new test strip.
E-2Meter malfunction. Contact customer service.
E-3Temperature too high or too low. Move to an area with a temperature between 50°F and 104°F (10°C and 40°C) and retest.
E-4Test strip is damaged or was not properly inserted. Retest with a new test strip.
E-5Blood sample was applied before the flashing blood drop symbol appeared. Retest with a new test strip.
E-6Not enough blood was applied to the test strip. Retest with a new test strip.
E-7Control solution test result is out of range. Repeat the control solution test.
E-8Meter malfunction. Contact customer service.
E-9Test strip error. Retest with a new test strip.
E-10Meter malfunction. Contact customer service.
E6Occurs when the temperature is outside thetemperature operating range
E5Occurs when an abnormal sample is detected,the contact bars of the test strips are dirty, oran incorrect sample type is used
E0Occurs when measurement of blood sample isattempted during QC lockout
Control Solution FlagMeter displays control flag when controlsolution is tested
Temperature FlagOccurs alongside E6 when the temperature isoutside the temperature operating range

For each error code/flag listed in Table 7, the appropriate conditions that trigger the error code/flag were tested and the error code/flag was verified. Additionally, conditions that should not trigger the error code/flag were also tested and the absence of the error code/flag was verified.

SHORT SAMPLE DETECTION

Blood glucose measurement from short samples of reduced sample volume) can lead to inaccurate results. To avoid the risk of inaccurate results, Blood Glucose Monitoring Systems should be able to detect that a short blood sample has been applied to the test strip and should not provide a result.

Three lots of Assure Titanium test strips were tested with venous blood samples at three glucose levels (50-65. 100-120, 200-250 mg/dL) that were verified using the YS1 2300. Approximately 0.1. 0.3. 0.5. 1. 5. or 10 uL of sample was applied to the test strip. Once the countdown was completed (if initiated), an error or the test result displayed on the meter.

The results demonstrated that the Assure Titanium Blood Glucose Monitoring System will either produce an error or an accurate result when short sampled.

SAMPLE PERTURBATION

Sample perturbation occurs when an appropriate volume of blood is applied to the test strip for glucose measurement, but an event such as wicking of blood away from the test strip, flicking of the test strip or flipping of the meter occurs during the start of the measurement and potentially alters the volume of the initial sample application.

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Three lots of Assure Titanium test strips were tested with venous blood samples at three glucose levels (50-65, 100-120, 200-250 mg/dL) that were verified using the YSI 2300. The samples were applied to the test strip and then perturbed by flicking the test strip, flipping the meter on theside, and wicking the sample away using a tissue.

The results demonstrated that the Assure Titanium Blood Glucose Monitoring System either provides an error or an accurate result when the sample is perturbed.

TESTING WITH USED TEST STRIP

The Assure Titanium Blood Glucose Monitoring System is designed to automatically detect the insertion of used Assure Titanium Blood Glucose Test Strips into the Assure Titanium Blood Glucose Meter. Insertion of used Assure Titanium Blood Glucose Test Strips into the system should not provide glucose measurement results.

Three lots of Assure Titanium test strips were dosed with either capillary finger-stick blood or control solution. The used test strips were then re-inserted into Assure Titanium meters to produce an error. The target acceptance criteria are as follow: All used test strips shall produce the E1 error upon re-insertion. All test results met the acceptance criteria.

SUMMARY OF CLINICAL STUDY (METHOD COMPARISON)

The Assure Titanium Blood Glucose Meter and Assure Titanium Blood Glucose Test Strips for the Assure Titanium Blood Glucose Monitoring System were tested in a multi-center study conducted at one (1) nursing/skilled nursing facility and two (2) endocrinology clinics to demonstrate clinical performance with patients/subjects in whom routine glucose monitoring is done in these settings.

Nursing/skilled nursing facility (Site #1) is a 475-bed institution in the state of Massachusetts. The study took place across both long-term care nursing and temporary care/rehabilitation units. The study was conducted by Point-of-Care operators who took capillary blood samples from 130 patients . Patients in this study represented 390 medical conditions and 239 different medications. Site #2 is an endocrinology clinic located in the state of California. Point-of-Care operators took capillary blood samples from 165 patients in this study represented 18 medical conditions and 245 different medications. Site #3 is an endocrinology clinic located in the state of Georgia. Point-of-Care operators took capillary blood samples from 101 patients. Patients in this study comprised 61 medical conditions and 196 different medications.

In total, capillary blood samples from 396 patients were measured and the results were compared to the YSI Model 2300 Glucose Analyzer, a lab instrument (comparator method). The tables 8-12 below show differences in glucose values between the Assure Titanium Blood Glucose Meter and the YSI method.

The slope, correlation, intercept, standard error, and 95% confidence interval as shown in Table 8 represent a strong linear correlation between the Assure Titanium Blood Glucose Monitoring System and the YSI 2300 reference analyzer.

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Results obtained by healthcare professionals
Nursing/SkilledNursing FacilityEndocrinology Clinic
Slope0.980.98
Correlation 'r'0.990.99
Intercept3.242.83
Number of Samples130266
Range Tested29.0 - 405.5 mg/dL43.7 - 492.5 mg/dL

Table 8: Assure Titanium Blood Glucose Monitoring System Correlation

Table 9: Nursing/Skilled Nursing Facility Accuracy results for glucose concentrations <75 mg/dL:

Difference range between the trueblood glucose level and the AssureTitanium Blood GlucoseMonitoring System resultWithin±5 mg/dLWithin±10 mg/dLWithin±12 mg/dLWithin±15 mg/dLExceeds±15 mg/dL
The percent (and number) ofsamples for which the differencebetween the Assure Titanium BloodGlucose Meter and the YSIcomparator method were within thedifference range shown in the toprow1/1(100%)1/1(100%)1/1(100%)1/1(100%)0/1(0%)

Table 10: Nursing/Skilled Nursing Facility Accuracy results for glucose concentrations ≥75 mg/dL:

Difference range between the trueblood glucose level and the AssureTitanium Blood GlucoseMonitoring System resultWithin±5 %Within±10 %Within±12 %Within±15 %Within±20 %Exceeds±20 %
The percent (and number) ofsamples for which the differencebetween the Assure Titanium BloodGlucose Meter and the YSIcomparator method were within thedifference range shown in the toprow80/129(62%)122/129(94.6%)125/129(96.9%)128/129(99.2%)129/129(100%)0/129(0%)

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Table 11: Endocrinology Clinic Accuracy results for glucose concentrations <75 mg/dL:

Difference range between the trueblood glucose level and the AssureTitanium Blood GlucoseMonitoring System resultWithin$\pm$ 5 mg/dLWithin$\pm$ 10 mg/dLWithin$\pm$ 12 mg/dLWithin$\pm$ 15 mg/dLExceeds$\pm$ 15 mg/dL
The percent (and number) ofsamples for which the differencebetween the Assure Titanium BloodGlucose Meter and the YSIcomparator method were within thedifference range shown in the toprow17/26(65.4%)25/26(96.2%)26/26(100%)26/26(100%)0/26(0%)

Table 12: Endocrinology Clinic Accuracy results for glucose concentrations ≥75 mg/dL:

Difference range between the trueblood glucose level and the AssureTitanium Blood GlucoseMonitoring System resultWithin$\pm$ 5 %Within$\pm$ 10 %Within$\pm$ 12 %Within$\pm$ 15 %Within$\pm$ 20 %Exceeds$\pm$ 20 %
The percent (and number) ofsamples for which the differencebetween the Assure Titanium BloodGlucose Meter and the YSIcomparator method were within thedifference range shown in the toprow163/240(67.9%)224/240(93.3%)230/240(95.8%)235/240(97.9%)240/240(100%)0/240(0%)

Medications and Medical Conditions:

The clinical studies included patients representative of each clinical setting. A detailed analysis of medical conditions and medications was performed on the clinical data set from all three (3) clinical study sites to identify any potential safety issues with the use of the Assure Titanium Blood Glucose Monitoring System within the intended use population. A frequency distribution table (Table 13) for each Drug Class as well as a table for medical conditions (Table 14) are presented below.

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Table 13: Medications Table
Assure Titanium Method Comparison Study Drug Table
Therapeutic Drug Class 2Therapeutic Drug Class 3NumberofPatients
Agents Acting on the Renin-Angiotensin SystemACE Inhibitors, Plain107
Agents Acting on the Renin-Angiotensin SystemAngiotensin II Receptor Blockers(ARBs), Plain62
All Other Therapeutic ProductsAngiotensin II Receptor Blockers(ARBs), Combinations11
All Other Therapeutic ProductsAll Other Therapeutic Products13
Anabolic Agents for Systemic UseOther Mineral Supplements29
AnalgesicsOpioids66
AnalgesicsOther Analgesics and Antipyretics132
AnestheticsAnesthetics, General1
AnestheticsAnesthetics, Local23
Antianemic PreparationsIron Preparations32
Antianemic PreparationsVitamin B12 and Folic Acid42
Antibacterials for Systemic UseBeta-Lactam Antibacterials,Penicillins13
Antibacterials for Systemic UseOther Antibacterials8
Antibacterials for Systemic UseOther Beta-Lactam Antibacterials5
Antibacterials for Systemic UseQuinolone Antibacterials7
Antibacterials for Systemic UseSulfonamides6
Antibacterials for Systemic UseTetracyclines1
Antibiotics and Chemotherapeuticsfor Determatological UseAntibiotics for Topical Use2
Antibiotics and Chemotherapeuticsfor Determatological UseChemotherapeutics for Topical Use1
Antidiarrheals, IntestinalInflammatory/Antiinfective AgentsAntidiarrheal Microorganisms10
Antidiarrheals, IntestinalInflammatory/Antiinfective AgentsAntipropulsives7
Antidiarrheals, IntestinalInflammatory/Antiinfective AgentsIntestinal Adsorbents1
Antidiarrheals, IntestinalInflammatory/Antiinfective AgentsIntestinal Antiinflammatory Agents35
Antidiarrheals, IntestinalInflammatory/Antiinfective AgentsOther Antidiarrheals2
Antiemetics and AntinauseantsAntiemetics and Antinauseants19
AntiepilepticsAntiepileptics110
Antifungals for Dermatologic UseAntifungals for Systemic Use1
Antifungals for Dermatologic UseAntifungals for Topical Use23
Antigout PreparationsAntigout Preparations21
Antihistamines for Systemic UseAntihistamines for Systemic Use24
AntihypertensivesAntiadrenergic Agents, GenerallyActing5
Antiadrenergic Agents, PeripherallyActing6
Arteriolar Smooth Muscle, AgentsActing on8
Other Antihypertensives1
Antiinflammatory and AntirheumaticProductsAntiinflammatory and AntirheumaticProducts, Non-Steroids34
Antimycotics for Systemic UseAntimycotics for Systemic Use5
Antiobesity Preparations, Excl. DietProductsAntiobesity Preparations, Excl. DietProducts3
Anticholinergic Agents2
Anti-Parkinson DrugsDopaminergic Agents16
Agents Against Amoebiasis and OtherProtozoal Diseases1
AntiprotozoalsAntimalarials5
Antiseptics and DisinfectantsAntiseptics and Disinfectants1
Antithrombotic AgentsAntithrombotic Agents237
Antivirals for Systemic useDirect Acting Antivirals5
Beta Blocking Agents147
Beta Blocking AgentsBeta Blocking Agents and Thiazides1
Bile and Liver TherapyBile Therapy1
Blood Substitutes and PerfusionSolutionsIrrigating Solutions4
Selective Calcium Channel Blockerswith Direct Cardiac Effects16
Calcium Channel BlockersSelective Calcium Channel Blockerswith Mainly Vascular Effects64
Calcium HomeostasisAnti-Parathyroid Agents1
Parathyroid Hormones and Analogues1
Antiarrhythmics, Class I and III10
Cardiac Glycosides6
Cardiac TherapyCardiac Stimulants, Excl. CardiacGlycosides4
Other Cardiac Preparations5
Vasodilators Used in Cardiac Diseases11
Corticosteroids for Systemic useCorticosteroids for Systemic use,Plain4
Corticosteroids, DermatologicalPreparationsCorticosteroids, Plain10
Cough Suppressants, Excl.Combinations with Expectorants16
Cough and Cold PreparationsExpectorants, Excl. Combinationswith Cough Suppressants10
DiureticsAldosterone Antagonists and OtherPotassium-Sparing Agents20
High-Ceiling Diuretics53
Low-Ceiling Diuretics, Excl.Thiazides3
Low-Ceiling Diuretics, Thiazides45
Drugs for Acid Related DisordersAntacids1
Drugs for Peptic Ulcer and Gastro-Oesophageal Reflux Disease (GORD)133
Drugs for ConstipationDrugs for Constipation307
Belladonna and Derivatives, Plain4
Drugs for Functional GastrointestinalDisordersDrugs for Functional GastrointestinalDisorders21
Propulsives7
Adrenergics, Inhalants53
Drugs for Obstructive AirwayDiseasesOther Drugs for Obstructive AirwayDiseases, Inhalants54
Other Systemic Drugs for ObstructiveAirway Diseases15
Drugs for Treatment of Bone DiseasesDrugs Affecting Bone Structure andMineralization12
Drugs Used in DiabetesBlood Glucose Lowering Drugs, Excl.Insulins359
Insulins and Analogues347
Ectoparasiticides, Incl. Scabicides,Insecticides, and RepellentsEctoparasiticides, Incl. Scabicides1
Emollients and ProtectivesEmollients and Protectives1
Endocrine TherapyHormone Antagonists and RelatedAgents3
Hormones and Related Agents5
Gynecological Antiinfectives andAntisepticsAntiinfectives and Antiseptics, Excl.Combinations with Corticosteroids4
ImmunosuppressantsImmunosuppressants12
Lipid Modifying AgentsLipid Modifying Agents, Plain263
Calcium20
Mineral SupplementsOther Mineral Supplements29
Potassium24
Muscle RelaxantsMuscle Relaxants, Centrally ActingAgents11
Nasal PreparationsDecongestants and Other NasalPreparations for Topical Use7
Antiinfectives3
Antiinflammatory Agents5
Decongestants and Antiallergics8
Ocular Vascular Disorder Agents1
Other Ophthalmologicals1
Other Alimentary Tract andMetabolism ProductsOther Alimentary Tract andMetabolism Products4
Other Mineral Supplements29
Other Dermatological PreparationsOther Dermatological Preparations2
Other Drugs for Disorders of theMusculo-Skeletal SystemOther Drugs for Disorders of theMusculo-Skeletal System1
Drugs Used in Addictive Disorders3
Other Nervous System DrugsOther Nervous System Drugs1
Parasympathomimetics1
Pituitary and Hypothalamic Hormonesand AnaloguesPosterior Pituitary Lobe Hormones1
Preparations for Treatment of Woundsand UlcersCicatrizants1
Antipsychotics16
PsycholepticsAnxiolytics48
Hypnotics and Sedatives58
Anti-Dementia Drugs12
Antidepressants147
PsychonalepticsPsycholeptics and Psychanaleptics inCombination8
Psychostimulants, Agents Used forADHD and Nootropics13
Androgens6
Estrogens13
Sex Hormones and Modulators of theGenital SystemHormonal Contraceptives forSystemic Use8
Other Sex Hormones and Modulatorsfo the Genital System2
Progestogens3
Progestogens and Estrogens inCombination1
Stomatological PreparationsStomatological Preparations1
Throat PreparationsThroat Preparations2
Thyroid TherapyAntithyroid Preparations6
Thyroid Preparations96
TonicsOther Mineral Supplements29
Topical Products for Joint andTopical Products for Joint and3
UrologicalsDrugs Used in Benign ProstaticHypertrophy40
Urologicals18
VasoprotectivesAgents for Treatment of Hemorrhoidsand Anal Fissures for Topical Use23
Capillary Stabilizing Agents1
VitaminsAscorbic Acid (Vitamin C), Incl.Combinations19
Calcium20
Multivitamins, Combinations44
Other Plain Vitamin Preparations6
Vitamin A and D, Incl. Combinationsof the Two123
Vitamin B1, Plain and in Combinationwith Vitamin B6 and B127
Vitamin B-Complex, Incl.Combinations1

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Table 14: Medical Conditions Table

Titanium Method Comparison Medical Conditions Table
ConditionCategoryConditionSubjectsw/MedicalCondition
AuditoryExcessive Cerumen3
Hard of Hearing19
Ruptured Eardrum1
BiliaryCholangitis3
Cholecystectomy3
Cholecystitis1
Cholelithiasis2
CancerBenign Neoplasm1
Bone Cancer1
Brain Cancer1
Breast Cancer8
Breast Cancer, Historical4
Colon Cancer1
Colon Cancer, Historical2
Hepatocellular Carcinoma1
Leukemia1
Lipoma2
Liver Cancer1
Lung Cancer2
Lymphatic Cancer1
Meningioma2
Multiple Myeloma1
Non-Hodgkin's Lymphoma1
Pancreatic Cancer1
Prostate Cancer6
Renal Cancer4
Renal Cancer, Historical1
Skin Cancer4
Skin Cancer, Historical3
Throat Cancer1
Thyroid Cancer2
Thyroid Cancer, Historical1
Aortic Aneurysm3
Aortic Insufficiency1
Aortic Regurgitation1
Aortic Stenosis9
Arrhythmia2
Arteriosclerosis2
Atrial Fibrillation32
Bradycardia5
Bundle Branch Block3
Cardiovascular Disease3
Carotid Artery Occlusion1
Chest Pain1
Chronic Systolic Heart Failure1
CardiacCoronary Artery Disease35
Elevated Troponin2
Endocarditis2
Heart Block2
Heart Disease7
Heart Failure41
Heart Murmur3
Hypertension248
Hypotension4
Mitral Insufficiency1
Mitral Regurgitation2
Mitral Stenosis1
Mitral Valve Prolapse2
Myocardial Infarction3
Myocarditis1
Occlusion and Stenosis of
Carotid Artery1
Palpitations2
Patent Foramen Ovale2
Premature Atrial Contraction1
Premature VentricularContractions1
Sick Sinus Syndrome1
Sinus Node Dysfunction1
Sleep Apnea19
Tachycardia4
Tricuspid Regurgitation1
Ventricular Arrhythmia1
Ventricular Outflow Obstruction1
Abscess3
DermatologicalAsteototic Dermatitis1
Blepharitis2
Dermatitis7
Eczema4
Erythema1
Foot Complications39
Intertrigo2
Pruritic Disorder1
Pruritus5
Psoriasis2
Rosacea2
Seborrheic Dermatitis3
Seborrheic Keratosis4
Skin Complications21
Skin Infection3
Skin Ulcer29
Tinea Pedis3
EndocrineTinea Versicolor1
Adrenal Insufficiency1
Adrenal Nodule1
Gastro-IntestinalGynecomastia1
Acid Reflux1
Celiac Disease1
Colitis2
Colon Polyp2
Constipation68
Diarrhea15
Diverticulitis1
Gastroenteritis1
Gastroesophageal RefluxDisease51
Gastrointestinal Hemorrhage2
Gastro-Intestinal Problems34
Heartburn1
Incontinence26
Indigestion2
Irritable Bowel Syndrome4
Irritable Colon1
Ischemic Colitis1
Malabsorption Syndrome1
Nausea6
Pancreatitis2
Peptic Ulcer3
Rectal Prolapse1
Ulcer1
Vomiting5
Bilirubinemia1
Cirrhosis1
Elevated INR1
Fatty Liver2
Hepatitis1
HepaticHyperbilirubinemia2
Hypoalbuminemia7
Liver Dysfunction2
Subtherapeutic INR1
Supratherapeutic INR1
Transaminitis1
HormonalHyperprolactinemia1
Hypogonadism3
Polycystic Ovarian Syndrome2
Arthritis12
Bacteremia1
ImmuneBacterial Infection3
Candida Infection3
Cellulitis2
Cold Sores1
Crohn's1
Eosinophilia1
Fever7
Gout19
Herpes Zoster1
Idiopathic Thrombocytopenic
Purpura1
Immunodeficiency1
Immunosuppression1
Joint Inflammation1
Leukocytosis16
Leukopenia1
Lichen Planus1
Lymphadenitis1
Lymphedema1
Myasthenia Gravis2
Myelodysplastic Syndrome1
Oligoarthritis1
Onychomycosis3
Paronychia1
Photosensitivity1
Plantar Fasciitis1
Polymyalgia Rheumatica2
Rheumatoid Arthritis10
Sepsis2
Septic Arthritis1
Sjogrens Syndrome1
Verruca1
Dyslipidemia11
Lipid DisorderHyperlipidemia126
Diabetic Ketoacidosis1
Gestational Diabetes1
Hemochromatosis1
Hereditary Coproporphyria1
Hypercalcemia4
Hypercholesteremia2
Hypercholesterolemia1
Hyperglycemia2
MetabolicHyperkalemia6
Hypernatremia3
Hyperuricemia1
Hypocalcemia1
Hypoglycemia5
Hypokalemia16
Hypomagnesemia6
Hyponatremia13
Malnutrition15
Microalbuminuria1
Obesity12
Tumoral Calcinosis1
Vitamin Deficiency42
Weight Gain5
Weight Loss19
Achalasia1
Bursitis3
Cervical Spondylosis3
Contracture1
Degenerative Disc Disease2
Degenerative Spondylolisthesis1
Dysarthria2
Dystonia1
Esophageal Dysmotility1
Hernia7
Kyphosis2
Leg Cramp1
Monoclonal Gammopathy ofUnknown Significance2
Muscle Spasms2
MusculoskeletalMuscle Weakness8
Muscular Dystrophy1
Myalgia1
Myositis1
Osteoarthritis36
Osteomyelitis6
Osteopenia3
Osteoporosis24
Pancytopenia3
Polyarthralgia2
Scoliosis2
Spinal Stenosis15
Temporomandibular JointSyndrome1
Tendinitis2
Agitation2
Alzheimer's14
NeurologicalAmnesia1
Anorexia4
Anxiety27
Autonomic Neuropathy1
Bipolar Disorder2
Carpal Tunnel3
Cervicalgia1
Chronic Hypomanic1
Chronic Pain60
Claustrophobia1
Cognitive Impairment29
Concussion1
Cord Compression1
Delirium12
Delusions2
Dementia32
Depression45
Diplegia1
Dizziness6
Dysautonomia1
Dysphagia27
Dysphonia1
Dysthymia1
Encephalopathy12
Essential Tremor2
Excoriation1
Fibromyalgia2
Globus Sensation1
Hallucinations2
Headache1
Lumbar Radiculopathy2
Memory Issues2
Migraine2
Mood Disorder1
Motor Skill Impairment11
Myoclonus1
Narcolepsy1
Neuralgia2
Neurologic Neglect Syndrome1
Neuropathy98
Nicotine Addiction1
Obsessive Compulsive Disorder1
Orthostatic Hypertension1
Orthostatic Hypotension11
Palsy1
Panic Attacks1
Parkinson's7
Polyneuropathy4
Post-Traumatic Stress Disorder1
Postural OrthostaticTachycardia Syndrome1
Radicular Syndrome1
Schizophrenia1
Sciatica2
Seizures4
Somnolence1
Syrinx of Spinal Cord1
Tardive Dyskinesia1
Tremor1
Vascular Dementia3
Vasovagal Syncope1
Vertigo4
NoneNone50
Blindness10
Bullous Keratopathy1
Cataract15
Conjunctivitis4
Dry Eye7
OcularGlaucoma22
Macular Degeneration6
Macular Edema1
Pseudophakia2
Retinopathy1
Vision Problems78
Visual Impairment1
Dental Abscess1
OralGingival Bleeding1
Xerostomia4
Abnormal Gait26
Apathy1
Ascites1
Balance Issues45
Debility53
Dehydration3
Fatigue3
OtherFrailty Syndrome4
Hyperproteinemia1
Hypothermia1
Impaired Mobility1
Insomnia53
Lethargy4
Mobility Issues1
Organ Prolapse3
Overweight1
Polypharmacy6
Polytrauma1
Sequela1
PancreaticIntraductal Papillary MucinousNeoplasm1
Acute Kidney Disease28
Chronic Kidney Disease53
Diabetes Insipidus1
Elevated Alkaline Phosphatase1
Elevated Uric Acid1
Hematuria6
Hepatorenal Syndrome1
RenalHyperosmolality1
Interstitial Nephritis1
Kidney Disease49
Nephrolithiasis1
Nephropathy1
Renal Mass1
Tubulointerstitial Disease1
Benign Prostatic Hyperplasia17
ReproductiveEndometriosis1
Enlarged Prostate2
Impotence25
Prostatitis1
Allergic Rhinitis6
Asthma12
Bronchiectasis2
Bronchitis1
Chronic Obstructive PulmonaryDisease10
Cough17
Dyspnea2
Hypercarbia2
RespiratoryLung Disease1
Pickwickian Syndrome1
Pleural Effusion2
Pneumonia13
Pneumonitis1
Pulmonary Edema5
Pulmonary Embolism1
Pulmonary Fibrosis1
Pulmonary MycobacterialInfection
Pulmonary Nodules2
Respiratory Failure9
Restrictive Lung Disease1
Shortness of Breath1
Sinusitis2
Upper Respiratory Infection1
Diaphoresis1
Goiter6
ThyroidHashimoto's4
Hyperparathyroidism4
Hyperthyroidism5
Hypoparathyroidism1
Hypothyroidism60
Thyroid Disorder2
Thyroid Nodule5
Bacteriuria1
Bladder Diverticulus1
Cystitis1
Dysuria6
UrinaryFrequent Urination3
Overactive Bladder5
Urinary Retention15
Urinary Tract Infection25
Anemia65
Aneurysm4
Arterial Embolism1
Artery Stenosis2
Atherosclerosis3
Cerebral Artery Syndrome1
Cerebral Microvascular Disease1
Cerebral Vascular Accident9
Cerebrovascular Disease3
VascularCoagulopathy2
Contusion3
Deep Vein Thrombosis3
Edema33
Epistaxis1
Gangrene2
Hematoma4
Hemorrhage6
Hemorrhoids11
Hypoxemia6
Hypoxia6
Ischemia1
Methemoglobinemia1
Peripheral Vascular Disease16
Pulmonary Vascular Congestion1
Purpura2
Stroke30
Swelling3
Syncope1
Thalassemia1
Thrombocytopenia14
Thrombocytosis4
Thrombosis1
Transient Ischemic Attack3
Varicose Veins1
Venous Insufficiency5
Venous Ulcer2

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ACCURACY AT EXTREMES

An Accuracy at Extremes study was executed to conduct a more robust evaluation between Assure Titanium Blood Glucose Monitoring System and the Yellow Springs Instrument (YSI) 2300 reference analyzer in the extreme upper and lower claimed blood glucose measuring range using a POC operator.

Capillary samples were collected and allowed to glycolyze or were spiked with high concentration glucose solution to acquire 100 samples for testing with the Assure Titanium Blood Glucose Monitoring System and the comparator: YSI 2300. There were 50 samples with glucose <80 mg/dL and 50 with glucose >300 mg/dL.

The data for the Accuracy at Extremes Glucose Values study are presented in Table 15 below.

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Glucose Concentrations <80 mg/dL
Within±5 mg/dLWithin ±10mg/dLWithin ±12 mg/dLWithin ±15mg/dLExceeds ±15mg/dL
43/50 (86%)49/50 (98%)50/50 (100%)50/50 (100%)0/50 (0%)
Glucose Concentrations >300 mg/dL
Within ±5%Within ±10%Within ± 12%Within ±15%Within ±20%Exceeds ±20%
32/50 (64%)46/50 (92%)50/50(100%)50/50(100%)50/50 (100%)0/50 (0%)

Table 15: Accuracy at extreme glucose value results for Assure Titanium Blood Glucose Monitoring System vs YSI 2300 Reference Analyzer

The results from this study demonstrated the proposed Assure Titanium Blood Glucose Monitoring System provides highly accurate glucose results when testing in the extreme glucose ranges.

USABILITY

A POC operator evaluation study was conducted during the clinical trial to evaluate the ease of use/understanding of the Assure Titanium Blood Glucose Monitoring System. The POC operator study involved POC operators filling out two usability questionnaires; one concerning the ease of use of the blood glucose meter system and one the ease of understanding of the Assure Titanium User Manual and the Quick Reference Guide (QRG). All the responses (100% combined) were positive i.e., Very Easy, or OK and indicated that the Assure Titanium Blood Glucose Monitoring System is easy to use. The overall rating of understanding the Quick ReferenceGuide and User Manual for Assure Titanium Blood Glucose Monitoring System was positive with participants rating 100% in categories of Very Easy, Easy or OK (combined).

Expected Values

Expected values for non-diabetics Expected blood glucose values for non-pregnant adults without diabetes l Fasting* <100 mg/dL 2 hours after meals <140 mg/dL

*Fasting is defined as no caloric intake for at least eight hours. Consult the patient's physician to determine the range that is appropriate for your patients.

  1. American Diabetes Association. Standards of medical care in diabetes- 2021. Diabetes Care. 2021:44(1)pS17."

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8. Proposed Labeling

Labeling adequately communicates device intended use, safety precautions and directions for use. It satisfies 21 CFR 809.10 requirements for in vitro diagnostic devices.

9. Conclusion

The information provided in this 510(k) premarket notification supports the Assure Titanium Blood Glucose Monitoring System is substantially equivalent to the predicate, StatStrip Glucose Hospital Meter System.

§ 862.1345 Glucose test system.

(a)
Identification. A glucose test system is a device intended to measure glucose quantitatively in blood and other body fluids. Glucose measurements are used in the diagnosis and treatment of carbohydrate metabolism disorders including diabetes mellitus, neonatal hypoglycemia, and idiopathic hypoglycemia, and of pancreatic islet cell carcinoma.(b)
Classification. Class II (special controls). The device, when it is solely intended for use as a drink to test glucose tolerance, is exempt from the premarket notification procedures in subpart E of part 807 of this chapter subject to the limitations in § 862.9.