K Number
K191657
Date Cleared
2020-03-20

(273 days)

Product Code
Regulation Number
862.1345
Reference & Predicate Devices
Predicate For
AI/MLSaMDIVD (In Vitro Diagnostic)TherapeuticDiagnosticis PCCP AuthorizedThirdpartyExpeditedreview
Intended Use

The Bioland Blood Glucose Monitoring System is comprised of Bioland Blood Glucose Meter and Bioland Blood Glucose Test Strips.

The Bioland Blood Glucose Monitoring System is intended to be used for the quantitative measurement of glucose (sugar) in fresh capillary whole blood samples drawn from the fingertip. The Bioland Blood Glucose Monitoring System is intended to be used by a single person and should not be shared. It is intended for self-testing outside the body (in vitro diagnostic use) by people with diabetes at home as an aid to monitor the effectiveness of diabetes control. The Bioland Blood Glucose Monitoring System should not be used for the diagnosis of or screening for diabetes. The Bioland Blood Glucose Monitoring System is not for use in neonates.

Device Description

The Bioland Blood Glucose Monitoring System (hereafter as the System) is an in vitro diagnostic device intended for the measurement of glucose in capillary blood. The System consists of the Bioland Blood Glucose Meter, Bioland Blood Glucose Test Strips, lancing device / sterile lancet and Bioland Glucose Control Solution. The Bioland Glucose Control Solution is for use with the Bioland Blood Glucose Meter and Bioland Blood Glucose Test Strips as a quality control check to verify the accuracy of blood glucose test results. These products have been designed and tested to work together as a system to produce accurate blood glucose test results. The measured result can be transferred to the appointed equipment that has been connected via the Bluetooth function.

AI/ML Overview

The Bioland Blood Glucose Monitoring System is a device intended for the quantitative measurement of glucose in fresh capillary whole blood samples drawn from the fingertip. It is for self-testing by people with diabetes at home.

Here's a breakdown of the acceptance criteria and the study that proves the device meets them:

1. Acceptance Criteria and Reported Device Performance

The acceptance criteria for this device are based on the FDA Guideline "Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use" issued on October 11, 2016, and relevant CLSI and ISO standards. The studies evaluate various performance aspects of the system.

Study/TestAcceptance CriteriaReported Device Performance and Compliance
Altitude StudyIndividual bias < ±10% compared with the mean value of Glucose Analyzer YSI 2300. SD < 5.0 mg/dL for glucose < 100 mg/dL, and CV < 5.0% for glucose ≥ 100 mg/dL.Individual bias < ±10%. SD < 5.0 mg/dL for glucose < 100 mg/dL. CV < 5.0% for glucose ≥ 100 mg/dL. Meets acceptance criteria. No significant altitude effects from 298 feet (91 m) to 10,744 feet (3,275 m).
Detection Limit EvaluationDisplay "Lo" when sample glucose < 20 mg/dL. Display "Hi" when sample glucose > 600 mg/dL.Displays "Lo" for glucose < 20 mg/dL. Displays "Hi" for glucose > 600 mg/dL. Meets acceptance criteria.
Hematocrit Interference StudyMean differences < ±10 mg/dL at glucose < 100 mg/dL. Mean differences < ±10% at glucose ≥ 100 mg/dL. All SD and CV within 5.0 mg/dL and 5.0% respectively.Mean differences within ±10 mg/dL (glucose < 100 mg/dL) and within ±10% (glucose ≥ 100 mg/dL) for Hct 20% to 60%. All SD and CV within 5.0 mg/dL and 5.0%. Meets acceptance criteria. Hct range from 20% to 60% is available.
Interference Substances EvaluationNo significant interference from specific endogenous and exogenous substances at tested concentrations. The % difference between test and control samples should be within acceptable limits.Highest concentration tested at which no significant interference was observed for 24 substances (e.g., Acetaminophen ≤ 8.0 mg/dL, Ascorbic Acid ≤ 5.0 mg/dL, Bilirubin ≤ 90 mg/dL, etc.). Implies adherence to acceptance criteria for the tested substances.
Linearity EvaluationCorrelation coefficient (r) > 0.95. 100% of individual glucose results bias fall within ±10% or ±10 mg/dL (comparison to a reference method like YSI 2300).Correlation coefficient r = 0.9998 (device G-427B and YSI 2300 highly correlated). 100% of individual bias within ±10% or ±10 mg/dL. Meets acceptance criteria. Linearity acceptable between 40 to 600 mg/dL.
Precision TestingCV within 5% at glucose concentration ≥ 100 mg/dL. SD within 5 mg/dL at glucose concentration < 100 mg/dL for both within-run and within-day precision.Within-run: Pooled CV for 3 lots: 6.5% (30-50 mg/dL), 3.5% (51-110 mg/dL), 3.6% (111-150 mg/dL), 3.4% (151-250 mg/dL), 3.3% (251-400 mg/dL). Pooled SD: 2.6 (30-50 mg/dL). Within-day: Pooled CV for 3 lots: 5.0% (30-50 mg/dL), 2.9% (51-110 mg/dL), 3.6% (111-150 mg/dL), 2.3% (151-250 mg/dL), 2.0% (251-400 mg/dL). Pooled SD: 2.0 (30-50 mg/dL). Meets acceptance criteria.
Robustness Validation TestAppearance and structures of meters normal after cleaning/disinfection. Individual bias for accuracy tests < ±10 mg/dL for glucose < 75 mg/dL and < ±10% for glucose > 75 mg/dL.Meters normal after 3,650 cycles of cleaning/disinfection. Individual bias < ±10 mg/dL (glucose < 75 mg/dL) and < ±10% (glucose > 75 mg/dL). Meets acceptance criteria.
Short Volume Detection TestingDevice detects insufficient blood volume and does not start measurement.Device detects insufficient blood volume (blood volume < 0.7uL) and the 5-second countdown does not start. Meets acceptance criteria. Required sample volume ≥ 0.7uL.
System Operation Conditions TestingBias or bias % < 10 mg/dL or 10%. CV/SD < 5.0% / 5.0 mg/dL.Bias or bias % < 10 mg/dL or 10%. CV/SD < 5.0% / 5.0 mg/dL. Meets acceptance criteria. Operating conditions: 50°F-104°F (10°C-40°C), 15%-85% RH.
Equipment Temperature Exposure Limits (Storage)Function and repeatability of meters after exposure to extreme temperatures (-4°F to 131°F) fall within acceptance criteria.Meters function and repeatability meet acceptance criteria after exposure to -4°F and 131°F. Meets EN ISO 15197:2015 requirements.
Equipment Humidity Exposure Limits (Storage)Function and repeatability of meters after exposure to extreme humidity (93±3% RH at 89.6°F) fall within acceptance criteria.Meters function and repeatability meet acceptance criteria after exposure to 93±3% RH at 89.6°F. Meets EN ISO 15197:2015 requirements.
Vibration and Shock TestFunction and repeatability after vibration and drop tests fall within acceptance criteria.Meters function and repeatability meet acceptance criteria after vibration and drop tests. Meets IEC 60068-2-64:2008, IEC 61010-1:2010, and EN ISO 15197:2015 requirements.
Shelf Life EvaluationSpecific stability periods for unopened and in-use vials of test strips and control solutions.Unopened test strips stable for 24 months, in-use (opened) for 90 days. Unopened control solutions stable for 12 months, in-use (opened) for 90 days. Meets ISO 23640:2011 guidelines.
Sample Perturbation StudySampling perturbation has no significant effect on precise blood glucose measurement.Results show no significant effect on precise blood glucose measurement from sampling perturbation.
Testing with Used StripsSystem does not provide measurement data and countdown does not activate when a used test strip is inserted.System does not provide measurement data and 5-second countdown does not activate when a used test strip is inserted.
User Performance Study (Lay User Study)Over 95% of individual bias ±15% for glucose concentration across the entire range. Lay users can operate the system properly.98.58% (346/351) of individual bias within ±15% for glucose concentration across the entire range. 100% (351/351) within ±20%. Lay users can operate the system properly after reading the user manual, even without guidance.
Satisfaction EvaluationMore than 85% of volunteers agree that labels and user manual provide enough information. More than 95% of volunteers' score higher than 80 points (for understanding materials).More than 85% agree labels/manual provide enough info. More than 95% scored >80 points. Indicates written materials provide sufficient understandable information for lay users.
Usability Engineering FileNo usability problems when validated under normal and worst-case scenarios.Evidence shows no usability problem when validated under normal conditions and worst-case scenarios.
Virucidal Efficacy (Disinfection)Pass acceptance criteria for HBsAg efficacy on main housing materials (ABS), test slot (PMMA), and LCD display lens (PC).Results passed acceptance criteria for all three parts of the subject device (ABS, PMMA, PC) using Clorox Germicidal Wipes.

2. Sample Sizes Used for the Test Set and Data Provenance

The document details sample sizes for various tests:

  • User Performance Study (Lay User Study): The test set included 351 individual glucose measurements.
  • Precision Testing (Within-run and Within-day): Tables provided show data for 3 lots of test strips, with measurements across 5 glucose concentration intervals. Although exact number of samples for each interval isn't explicitly stated, these tables typically involve multiple replicates (e.g., 10-20 replicates per lot per glucose concentration level). For example, it lists means, SDs, and CVs implying multiple measurements were taken.
  • Other tests (Altitude, Hematocrit, Linearity, Interference, etc.): The specific number of individual samples for these tests is generally not explicitly quantified in the provided summary. However, these are standard laboratory tests that involve a sufficient number of replicates and samples at various concentrations to ensure statistical validity, following guidelines such as CLSI EP7-A2, CLSI EP6-A, and FDA guidelines.
  • Data Provenance: The document does not explicitly state the country of origin of the data for these studies or whether they were retrospective or prospective. Given that Bioland Technology Ltd. is located in Shenzhen, Guangdong, People's Republic of China, it is highly probable that the studies were conducted in China. The studies are described as "Pre-clinical data" and "Summary of performance testing," suggesting they were specifically conducted prospectively for this 510(k) submission to demonstrate device performance.

3. Number of Experts and Qualifications for Ground Truth

The document does not mention the number of experts or their specific qualifications for establishing ground truth. Instead, it relies on a reference method, the Glucose Analyzer YSI 2300, which is a widely accepted and highly accurate laboratory instrument for determining blood glucose concentration. This method inherently serves as the "ground truth" comparator for the device's measurements.

4. Adjudication Method for the Test Set

No adjudication method (e.g., 2+1, 3+1) is mentioned in the context of human expert review of results, as the studies primarily rely on direct comparison to a laboratory reference standard (YSI 2300) and quantitative statistical analysis. For the user performance study, the acceptance criteria are numerical (e.g., % of results within a certain bias), not subjective expert judgment that would require adjudication.

5. Multi-Reader Multi-Case (MRMC) Comparative Effectiveness Study

An MRMC study was not conducted. This device is a self-monitoring blood glucose system, not an imaging AI system where human readers assist or benefit from AI. The user performance study evaluates the lay user's ability to operate the device correctly and obtain accurate results, which is a different type of assessment than an MRMC study.

6. Standalone Performance (Algorithm Only)

The device itself (Bioland Blood Glucose Monitoring System) is an "algorithm only" or "standalone" device in the sense that it provides a direct glucose measurement without human interpretation beyond reading the displayed number. The performance tests described (Precision, Linearity, Hematocrit, etc.) directly assess this standalone performance against a proven reference method (YSI 2300). The user performance study then adds the human-in-the-loop component, evaluating the system's performance when operated by lay users.

7. Type of Ground Truth Used

The primary ground truth used for the performance studies (e.g., Altitude, Hematocrit, Linearity, Precision) is comparison to a laboratory reference method, specifically the Glucose Analyzer YSI 2300. This is a highly accurate and precise instrument that measures glucose in blood samples. For the user performance study, the "ground truth" for the glucose values themselves would also be obtained from such a reference method, against which the lay user's device readings are compared.

8. Sample Size for the Training Set

This document describes a premarket notification (510(k)) for a medical device that measures blood glucose. It is not an AI/ML device that requires a "training set" in the sense of machine learning. The device's underlying "algorithm" is the electrochemical biosensor technology. Therefore, the concept of a "training set" for an algorithm, as seen in AI/ML, does not apply here. The data presented are from verification and validation studies for a traditional in vitro diagnostic device.

9. How the Ground Truth for the Training Set Was Established

As stated in point 8, the concept of a training set is not applicable to this type of device. The ground truth for the test and validation of the device's performance was established using a Glucose Analyzer YSI 2300 as the reference method.

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Image /page/0/Picture/0 description: The image shows the logo of the U.S. Food and Drug Administration (FDA). The logo consists of two parts: the Department of Health & Human Services logo on the left and the FDA logo on the right. The FDA logo features the letters "FDA" in a blue square, followed by the words "U.S. FOOD & DRUG ADMINISTRATION" in blue text.

March 20, 2020

Bioland Technology Ltd. % Ke-Min Jen No. A6B7 (Block G), ShangRong Industrial Zone No. 5 Baolong Road, Baolong Community, Longgang District, Shenzhen, CN 518116 Guangdong

Re: K191657

Trade/Device Name: Bioland Blood Glucose Monitoring System Regulation Number: 21 CFR 862.1345 Regulation Name: Glucose Test System Regulatory Class: Class II Product Code: NBW Dated: June 12, 2019 Received: June 21, 2019

Dear Ke-Min Jen:

We have reviewed your Section 510(k) premarket notification of intent to market the device referenced above and have determined the device is substantially equivalent (for the indications for use stated in the enclosure) to legally marketed predicate devices marketed in interstate commerce prior to May 28, 1976, the enactment date of the Medical Device Amendments, or to devices that have been reclassified in accordance with the provisions of the Federal Food, Drug, and Cosmetic Act (Act) that do not require approval of a premarket approval application (PMA). You may, therefore, market the device, subject to the general controls provisions of the Act. Although this letter refers to your product as a device, please be aware that some cleared products may instead be combination products. The 510(k) Premarket Notification Database located at https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfpmn/pmn.cfm identifies combination product submissions. The general controls provisions of the Act include requirements for annual registration, listing of devices, good manufacturing practice, labeling, and prohibitions against misbranding and adulteration. Please note: CDRH does not evaluate information related to contract liability warranties. We remind you, however, that device labeling must be truthful and not misleading.

If your device is classified (see above) into either class II (Special Controls) or class III (PMA), it may be subject to additional controls. Existing major regulations affecting your device can be found in the Code of Federal Regulations, Title 21, Parts 800 to 898. In addition, FDA may publish further announcements concerning your device in the Federal Register.

Please be advised that FDA's issuance of a substantial equivalence determination does not mean that FDA has made a determination that your device complies with other requirements of the Act or any Federal statutes and regulations administered by other Federal agencies. You must comply with all the Act's requirements, including, but not limited to: registration and listing (21 CFR Part 807); labeling (21 CFR Part 801 and Part 809); medical device reporting of medical device-related adverse events) (21 CFR

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  1. for devices or postmarketing safety reporting (21 CFR 4, Subpart B) for combination products (see https://www.fda.gov/combination-products/guidance-regulatory-information/postmarketing-safety-reportingcombination-products); good manufacturing practice requirements as set forth in the quality systems (OS) regulation (21 CFR Part 820) for devices or current good manufacturing practices (21 CFR 4, Subpart A) for combination products; and, if applicable, the electronic product radiation control provisions (Sections 531-542 of the Act); 21 CFR 1000-1050.

Also, please note the regulation entitled, "Misbranding by reference to premarket notification" (21 CFR Part 807.97). For questions regarding the reporting of adverse events under the MDR regulation (21 CFR Part 803), please go to https://www.fda.gov/medical-device-safety/medical-device-reportingmdr-how-report-medical-device-problems.

For comprehensive regulatory information about medical devices and radiation-emitting products, including information about labeling regulations, please see Device Advice (https://www.fda.gov/medicaldevices/device-advice-comprehensive-regulatory-assistance) and CDRH Learn (https://www.fda.gov/training-and-continuing-education/cdrh-learn). Additionally, you may contact the Division of Industry and Consumer Education (DICE) to ask a question about a specific regulatory topic. See the DICE website (https://www.fda.gov/medical-device-advice-comprehensive-regulatoryassistance/contact-us-division-industry-and-consumer-education-dice) for more information or contact DICE by email (DICE@fda.hhs.gov) or phone (1-800-638-2041 or 301-796-7100).

Sincerely.

Marianela Perez-torres -S

Marianela Perez-Torres, Ph.D. Acting Deputy Director Division of Chemistry and Toxicology Devices OHT7: Office of In Vitro Diagnostics and Radiological Health Office of Product Evaluation and Quality Center for Devices and Radiological Health

Enclosure

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Indications for Use

510(k) Number (if known) K191657

Device Name Bioland Blood Glucose Monitoring System

Indications for Use (Describe)

The Bioland Blood Glucose Monitoring System is comprised of Bioland Blood Glucose Meter and Bioland Blood Glucose Test Strips.

The Bioland Blood Glucose Monitoring System is intended to be used for the quantitative measurement of glucose (sugar) in fresh capillary whole blood samples drawn from the fingertip. The Bioland Blood Glucose Monitoring System is intended to be used by a single person and should not be shared. It is intended for self-testing outside the body (in vitro diagnostic use) by people with diabetes at home as an aid to monitor the effectiveness of diabetes control. The Bioland Blood Glucose Monitoring System should not be used for the diagnosis of or screening for diabetes. The Bioland Blood Glucose Monitoring System is not for use in neonates.

Type of Use (Select one or both, as applicable)
Prescription Use (Part 21 CFR 801 Subpart D)Over-The-Counter Use (21 CFR 801 Subpart C)

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Image /page/3/Picture/0 description: The image contains the word "bioland" in blue font, followed by three Chinese characters. The characters are also in blue and appear to be part of the brand name. There is a small registered trademark symbol next to the "d" in "bioland" and another one next to the last Chinese character.

有 爱 有 快 乐

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen, Guangdong, People's Republic of China

5. 510(k) Summary (Per 21 CFR 807.92) 510(k) number: K191657

Type of 510(k) submissionTraditional
Basis for the submissionA New Device
Common Name of the ProposedDeviceSelf-Monitoring Blood Glucose Test Systems
Trade nameBioland Blood Glucose Monitoring System
510(k) submitterBioland Technology Ltd.No. A6B7 (Block G), ShangRong Industrial ZoneNo. 5 Baolong Road, Baolong Community, LongganDistrict 518116, Shenzhen, Guangdong, People'sRepublic of ChinaWebsite: http://www.bioland.com.cnEmail: bioland-c@bioland.com.cnPhone: +86-755-2896-1218Fax: +86-755-2896-2868
Registered Establishment Number3004101980
Owner number9057599
Date preparedMarch 17, 2020
Contact personDr. JEN, KE-MINTEL: +886-3-5208829 FAX: +886-3-5209783Email: ceirs.jen@msa.hinet.net
Preference for continuedconfidentiality (21 CFR 807.95)510(k) Summary
Classification regulationSYSTEM, TEST, BLOOD GLUCOSE, OVER THECOUNTER (21 CFR 862.1345)
Class2
PanelClinical Chemistry
Product codeNBW
Predicate DeviceManufacturer: Bioland Technology Ltd.Proprietary name: Bioland G-423 Blood Glucose Monitoring System510(k) number: K113077

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Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen. Guangdong. People's Republic of China

● Indications For Use:

The Bioland Blood Glucose Monitoring System is comprised of Bioland Blood Glucose Meter and Bioland Blood Glucose Test Strips.

The Bioland Blood Glucose Monitoring System is intended to be used for the quantitative measurement of glucose (sugar) in fresh capillary whole blood samples drawn from the fingertip. The Bioland Blood Glucose Monitoring System is intended to be used by a single person and should not be shared. It is intended for self-testing outside the body (in vitro diagnostic use) by people with diabetes at home as an aid to monitor the effectiveness of diabetes control. The Bioland Blood Glucose Monitoring System should not be used for the diagnosis of or screening for diabetes. The Bioland Blood Glucose Monitoring System is not for use in neonates.

● Device Description:

The Bioland Blood Glucose Monitoring System (hereafter as the System) is an in vitro diagnostic device intended for the measurement of glucose in capillary blood. The System consists of the Bioland Blood Glucose Meter, Bioland Blood Glucose Test Strips, lancing device / sterile lancet and Bioland Glucose Control Solution. The Bioland Glucose Control Solution is for use with the Bioland Blood Glucose Meter and Bioland Blood Glucose Test Strips as a quality control check to verify the accuracy of blood glucose test results. These products have been designed and tested to work together as a system to produce accurate blood glucose test results. The measured result can be transferred to the appointed equipment that has been connected via the Bluetooth function.

● Test Principle

Electrochemical biosensor with carbon electrodes that is referring to detection of glucose by the test strips. The test is based on the measurement of electrical current generated by the reaction of capillary whole blood glucose with glucose oxidase on the test strip. The meter measures the strength of the current which is proportional to the concentration of glucose present and displays the corresponding blood glucose level.

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Image /page/5/Picture/0 description: The image contains the word "bioland" in blue, followed by Chinese characters. The Chinese characters are also in blue and appear to be a translation or a related term to "bioland". The font is sans-serif and the overall design is simple and clean. There are two circled R symbols on the top right of the words.

有 爱 有 快 乐

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

● Comparison Table

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen, Guangdong, People's Republic of China

ComparisonItemsPredicate deviceSubject deviceVerdict
ManufacturerBioland Technology Ltd.Bioland Technology Ltd.Same
Device namemodel numberBioland Blood GlucoseMonitoring System, G-423Bioland Blood GlucoseMonitoring System, G-427BSimilar
Product CodeNBWNBWSame
510(k) NumberK113077K191657--
Indications forUse (IFU)Intended to be used for thequantitative measurement ofglucose in capillary whole bloodfrom the fingertip. It is intendedfor use by people with diabetesmellitus at home (Over-theCounter) as an aid in monitoringthe effectiveness of diabetescontrol program.Intended to be used for thequantitative measurement ofglucose in capillary whole bloodfrom the fingertip. It is intendedfor use by people with diabetesmellitus at home (Over-theCounter) as an aid in monitoringthe effectiveness of diabetescontrol program.Same
Test PrincipleElectrochemical biosensor withcarbon electrodes that isreferring to detection of glucoseby the test strips. The test isbased on the measurement ofelectrical current generated bythe reaction of capillary wholeblood glucose with glucoseoxidase on the test strip. Themeter measures the strength ofthe current which is proportionalto the concentration of glucosepresent and displays thecorresponding blood glucoselevel.Electrochemical biosensor withcarbon electrodes that isreferring to detection of glucoseby the test strips. The test isbased on the measurement ofelectrical current generated bythe reaction of capillary wholeblood glucose with glucoseoxidase on the test strip. Themeter measures the strength ofthe current which is proportionalto the concentration of glucosepresent and displays thecorresponding blood glucoselevel.Same
EnzymeGlucose oxidaseGlucose oxidaseSame
MeasuringmethodAmperometric technology usingglucose oxidaseAmperometric technology usingglucose oxidaseSame
DisplayLCDLCD with backlightSimilar
Specimen typeCapillary whole blood fromfingertipCapillary whole blood fromfingertipSame
Detecting Range20 ~ 600 mg/dL40 ~ 600 mg/dLSimilar
Operatingconditions50°F~104°F (10 °C ~ 40 °C)Humidity <90% RH50°F~104°F (10 °C ~ 40 °C)Humidity: 15%~85%RHSimilar
Storage/Transportationcondition(System: Meter& Test Strips)39.2°F ~104°F (4 °C ~ 40 °C)Humidity <85% RH39.2°F104°F(4 °C 40 °C)Humidity: 10% - 85% RHSimilar
Storage/Transportationcondition(Meter)-4°F~131°F(-20°C ~ 55 °C)Humidity < 93±3% RH700hPa ~ 1060hPa-4°F131°F(-20°C ~ 55 °C)Humidity < 93±3% RH700hPa 1060hPaSame
TemperaturecompensationmechanismAutomatic compensation withbuilt-in thermistorAutomatic compensation withbuilt-in thermistorSame
Maximumoperatingaltitude10,744 feet10,744 feetSame
Test stripchemicalcomponent10% Glucose Oxidase (A. niger)50% Electron shuttle8% Enzyme protector32% Non-reactive ingredients10% Glucose Oxidase (A. niger)50% Electron shuttle8% Enzyme protector32% Non-reactive ingredientsSame
Meter Weight(w/o batteries)54.6g48.0 gDifferent
Test stripcalibrationUse code test strip to calibratethe meterNo Code function, no need tocalibrate the meterDifferent
Control solutionsTaiDoc Glucose ControlSolution,Level 1, Level 2, Level 3,cleared under K093724Bioland Glucose ControlSolutionLevel 1, Level 2, Level 3Different
Hct range15%~55%20% ~ 60%Different
Sample volume≥1.8 uL≥0.7 uLDifferent
Meter dimension81 (L) ×62 (W) x 19 (H) mm90 (L)×54 (W)×13 (H) mmDifferent
Battery power1 x CR2032 battery (DC 3V)DC 3V (AAA×2 alkalinebatteries)Different
Battery lifeAbout 1000 normal testsAbout 500 normal testsDifferent
Measuring Time10 sec6 secDifferent
Memory storage180 tests448 testsDifferent

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Image /page/6/Picture/0 description: The image shows the word "bioland" in blue, followed by the Chinese characters "爰奥乐" also in blue. There is a registered trademark symbol to the right of both words. The font is sans-serif and the text is left-aligned.

有 爱 有 快 乐

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen, Guangdong, People's Republic of China

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Image /page/7/Picture/0 description: The image shows the word "bioland" in blue, followed by three Chinese characters also in blue. The font is sans-serif and the text is horizontally aligned. There is a registered trademark symbol to the right of the word "bioland" and above the Chinese characters.

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen. Guangdong. People's Republic of China

● Substantial Equivalence (SE) Discussion

A claim of substantial equivalence is made to Bioland G-423 Blood Glucose Monitoring System, K113077. Both of them have the same indications for use, the same working principle and technologies, operating & storage conditions and detecting range. The major differences for both devices are Meter weight, Test strip calibration, Control solution, Hct Range, Sample volume, Meter dimension, Battery power, Battery life, Measuring time and Memory storage.

The meter weight without batteries for the subject device is 48.0 grams and is 54.6 grams for predicate device. The subject device is lighter than the predicate device. Since they are used at home, the lighter weight of the subject device will bring more convenience for the users. The difference of weights between subject device and predicate device is related to the manufacture's design characteristics, and it will not raise any new safety and effectiveness concerns for subject device.

The predicate device uses code test strip to calibrate the meter. The subject device provides No Code function, which means that the user does not need to calibrate the meter, making it easier for the user to monitor blood glucose level at home. That also means the subject device is more convenient to use than the predicate device, and the difference will not raise any new safety and effectiveness concerns for the subject device.

The control solutions are different brands. The control solutions of the predicate device is TaiDoc Glucose Control Solution, and the subject device is Bioland Glucose Control Solution. They are all labeled the same names as Level 1/Level 2/Level 3. Though the real glucose concentrations for Levels 1/2/3 are not the same, the user can use them for quality control purposes. The differences of control solutions will not raise any new safety and effectiveness concerns for the subject device.

The Hct ranges for the subject device and predicate device are different. The Hct range for the subject device is 20% to 60% and it is 15% to 55% for predicate device. As the effect of Hct range in the blood has been tested and validated, it is indicated in the labeling. The difference of Hct range will not raise any new safety and effectiveness concerns for the subject device.

The sample volume for the subject device is 0.7 uL and it is 1.8 uL for the predicate device. Since less sample volume means more convenient to the users at home, the short volume detection testing was performed and passed. The subject device will not bring any new safety and effectiveness concerns due to the less sample volume.

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Image /page/8/Picture/0 description: The image shows the word "bioland" in blue font, followed by three Chinese characters also in blue font. There is a registered trademark symbol to the right of the word "bioland" and another one to the right of the Chinese characters. The font is sans-serif and the overall image has a clean and professional look.

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen, Guangdong, People's Republic of China

The meter dimension of the predicate device is different from the subject device. This difference is due to the different design aspects, and not related to the safety and effectiveness concerns.

The battery power is one CR2032 (DC 3V) battery for the predicate device and 2 AAA alkaline (DC 3V) batteries for the subject device. Their resultant voltages of the electrical circuits are the same DC 3V, and the differences of battery types are related to the different electrical circuit design aspects, not related to the safety and effectiveness concerns. The batteries passed the electrical safety testing of IEC 61010-1:2010 and IEC 61010-2-101:2015 and the relevant bench performance testing. so there is no new safety and effectiveness concerns raised by the difference of batteries used.

The battery life is 500 measurements for the subject device and it is 1000 measurements for the predicate device. This is the direct result due to using the different batteries types. There is no new safely and effectiveness concerns raised by the different batteries used.

Measurement time for the subject device is 6 seconds and it is 10 seconds for the predicate device. The user of the subject device will get the measurement data faster than the predicate device. The subject device is more convenient or safer than the predicate device. There is no any new safety and effectiveness concerns raised by different measurement times for the subject device.

Memory storage for the subject device is 448 sets and it is 180 sets for the predicate device. Since the more memory storage means more space for storing the measurement data for the user's' reference. The difference of memory storages will not raise any new safety and effectiveness concerns for the subject device.

Besides, the subject device and the predicate device are intended for use in the quantitative measurement of glucose in fresh capillary whole blood drawn from the fingertips. The subject device complies with the IEC/EN 61010-1:2001 Safety requirements for electrical equipment for measurement, control and laboratory use -Part 1: general requirements and the IEC/EN 61010-2-101 2015 Safety requirements for electrical equipment for measurement, control and laboratory use – Part 2-101: Particular requirements for in vitro diagnostic (IVD) medical equipment. Thus, the differences listed above will not raise any new safety and effectiveness concerns for the subject device. The subject device is substantially equivalent to the predicate device K113077.

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Image /page/9/Picture/0 description: The image shows the word "bioland" in blue, followed by three Chinese characters, also in blue. There are two trademark symbols, one above the "d" in "bioland" and one above the last Chinese character. The font is sans-serif and the overall design is simple and modern.

有 爱 有 快

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen. Guangdong. People's Republic of China

Synopsis of Test Methods and Results

Pre-clinical data are employed upon submission of this 510(k) premarket notification according to the Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use, issued on October 11, 2016.

● Summary of performance testing

. Altitude study:

The testing results show the individual bias is less than ±10 % compared with the mean value of Glucose Analyzer YSI 2300 from altitude 298 feet (91 m) to 10,744 feet (3,275 m). The SD at blood glucose concentration < 100 mg/dL and CV at the blood glucose concentration ≥ 100 mg/dL for the measurements were less than 5.0 mg/dL and 5.0 %, respectively. The testing results met the acceptance criteria. It shows no significant altitude effects on the blood glucose measurement of the Bioland Blood Glucose Monitoring System G-427B from altitudes 298 feet to 10,744 feet (91 m to 3,275 m).

1 Detection limit evaluation report

The test results show that when the sample glucose value is lower than 20 mg/dL, the Bioland blood glucose meter will display "Lo", and when the sample glucose value is higher than 600 mg/dL, the Bioland blood glucose meter will display "Hi". These test results meet the acceptance criteria. The BGMS G-427B passes the detection limit evaluation.

. Hematocrit interference study:

According to the test results, the mean differences of the BGMS G-427B measurements fall within ± 10 mg/dL at glucose concentration < 100 mg/dL, and within ±10% at glucose concentration ≥ 100 mg/dL from Hct 20 % to Hct 60 %. All of SD and CV were within 5.0 mg/dL and 5.0% in this study respectively. The test results met the acceptance criteria. The Hct ranges from 20% to 60% were available for Bioland Blood Glucose Monitoring System G-427B.

. Interference substances evaluation:

This evaluation was conducted under the directions of FDA Guideline "Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use" issued on October 11, 2016 and CLSI EP7-A2. According to this study, evaluating 24 endogenous and exogenous substances in venous blood samples with 5 different levels of glucose concentrations, split into a control sample and a test sample.

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Image /page/10/Picture/0 description: The image shows the logo for Bioland. The word "bioland" is written in blue, lowercase letters. To the right of "bioland" are three Chinese characters, also in blue. There are two registered trademark symbols, one above the "d" in "bioland" and one above the last Chinese character.

有 爱 有 快

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen. Guangdong. People's Republic of China

The % difference between the test and control sample was calculated. The highest concentration tested at which no significant interference was summarized as below.

A sample with large amount of reducing substances such as Acetaminophen ( ≤ 8.0 mg/dL), Ascorbic Acid (≤5.0 mg/dL), Bilirubin (≤90 mg/dL), Cholesterol (≤500 mg/dL), Creatinine (≤5.0 mg/dL), Dopamine (≤2.0 mg/dL), EDTA (≤ 360 mg/dL), Galactose (≤900 mg/dL), Gentisic acid (≤ 5.0 mg/dL), Glutathione (≤53 mg/dL), Hemoglobin (≤500 mg/dL), Heparin (≤8000 U/dL), Ibuprofen (≤50 mg/dL), Icodextrin (≤13 mg/dL), L-dopa (≤10 mg/dL), Maltose (≤900 mg/dL), Methyldopa (≤3.0 mg/dL), Pralidoxime Iodide (≤25 mg/dL), Salicylate (≤60 mg/dL), Tolazamide (≤100 mg/dL), Tolbutamide (≤ 400 mg/dL), Triglycerides (≤ 2000 mg/dL), Uric Acid(≤ 8.0 mg/dL), Xylose (≤100 mg/dL).

. Linearity evaluation:

Linearity evaluation are conducted under the directions of CLSI EP6-A. According to our test results, the correlation coefficient r = 0.9998, which is greater than 0.95, that is to say, the device G-427B and YSI 2300 were highly correlated. The linearity of our measurement is acceptable between 40 to 600 mg/dL. 100 % of the bias of individual glucose results fall within ±10% or ±10 mg/dL. The test results met the acceptance criteria. The Bioland Blood Glucose Monitoring System G-427B passes the linearity testing.

1 Precision testing:

There are two parts, within-run and within-day testing, for the precision testing. The evaluation was conducted according to the FDA Guideline "Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use" issued on October 11, 2016. The precision evaluation results of Bioland Blood Glucose Monitoring System G-427B meet the acceptance criteria, i.e., CV within 5% at glucose concentration ≥ 100 mg/dL and SD within 5 mg/dL at glucose concentration < 100 mg/dL. The summary tables of within-run and within-day precision testing are shown as below. The test results meet the acceptance criteria, and Bioland Blood Glucose Monitoring System G-427B passes the precision evaluation.

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Image /page/11/Picture/0 description: The image shows the word "bioland" in blue, followed by three Chinese characters also in blue. The word "bioland" is in lowercase letters and has a registered trademark symbol after the "d". The Chinese characters are to the right of the word "bioland" and also have a registered trademark symbol after them. The background of the image is white.

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen, Guangdong, People's Republic of China

Glucose concentration interval (mg/dL)30-5051-110111-150151-250251-400
YSI Mean(mg/dL)40.389.9131.5199.3323.8
Mean (mg/dL)40.089.7130.8200.0322.1
Lot1SD2.73.14.67.211.1
CV(%)6.6%3.5%3.5%3.6%3.4%
Mean (mg/dL)40.290.2130.0199.9323.2
Lot2SD2.73.14.76.510.9
CV(%)6.7%3.5%3.6%3.3%3.4%
Mean (mg/dL)40.089.9130.7201.3320.9
Lot3SD2.53.14.67.010.1
CV(%)6.3%3.4%3.5%3.5%3.1%
Mean (mg/dL)40.189.9130.5200.4322.1
PooledSD2.63.14.66.910.7
CV(%)6.5%3.5%3.6%3.4%3.3%

Summary table of within-run precision testing with venous whole blood samples

Summary table of within-day precision testing with control solutions

Glucose concentration interval (mg/dL)30-5051-110111-150151-250251-400
YSI Mean(mg/dL)41.994.0131.1199.2325.3
Lot1Mean (mg/dL)40.190.5130.8193.0315.6
Lot1SD2.02.54.64.86.2
Lot1CV(%)5.1%2.8%3.5%2.5%2.0%
Lot2Mean (mg/dL)40.190.3130.0192.4314.2
Lot2SD2.02.74.74.66.2
Lot2CV(%)5.1%3.0%3.6%2.4%2.0%
Lot3Mean (mg/dL)40.089.8130.7192.1315.0
Lot3SD1.92.54.64.06.0
Lot3CV(%)4.9%2.8%3.5%2.1%1.9%
PooledMean (mg/dL)40.190.2130.5192.5314.9
PooledSD2.02.64.64.56.2
PooledCV(%)5.0%2.9%3.6%2.3%2.0%

. Robustness validation test:

This validation test was conducted under the directions of FDA Guideline "Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use" issued on October 11, 2016 and ISO 15197:2013. According to the test results, the appearance and structures of the meters were normal after 3,650 cycles of cleaning and disinfection. Also, based on the results of the accuracy test, all the individual

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Image /page/12/Picture/0 description: The image shows the word "bioland" in blue, followed by three Chinese characters also in blue. There is a small circle with an R in it above the "d" in "bioland" and above the last Chinese character. The font is sans-serif and the overall design is simple and clean.

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen, Guangdong, People's Republic of China

bias of the test results compared with YSI mean were less than ±10 mg/dL at glucose concentrations < 75 mg/dL and less than ± 10 % at glucose concentrations > 75 mg/dL. The test results meet the acceptance criteria. The Bioland Blood Glucose Monitoring System passes the Robustness validation study.

. Short volume detection testing:

Under the directions CLSI EP7-A2 and FDA Guideline "Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use" issued on October 11, 2016. The test results met the acceptance criteria by applying the blood volume ≥ 0.7uL. The volume of blood sample required at least 0.7uL for Bioland Blood Glucose Monitoring System G-427B. If the blood volume is not enough, the device will detect this shortage situation, and the 5-second countdown procedure is not to start.

. System operation conditions testing

The study was performed in accordance with guidelines of ISO 23640:2011 In vitro diagnostic medical devices — Evaluation of stability of in vitro diagnostic reagents. According to the test results, the bias or bias % was less than 10 mg/dL or 10% and CV/SD were less than 5.0% / 5.0 mg/dL. The test results met the acceptance criteria. So we can acclaim that the system operating conditions for the subject device are 50 °F - 104 °F, 15% - 85% RH.

. Equipment temperature exposure limits for storage testing

The study was performed in accordance with guideline of EN ISO 15197:2015, Section 5.11: Equipment temperature exposure limits for storage. According to the test results, the function and repeatability of the meters after being treated to extreme temperature, -4 °F or 131 °F fall within the acceptance criteria. That is, the meters could stand the extreme temperature (-4 °F to 131 °F), as required in EN ISO 15197:2015.

. Equipment humidity exposure limits for storage testing

The study was performed in accordance with guideline of EN ISO 15197:2015, Section 5.12: Equipment humiditv exposure limits for storage. According to the test results, the function and repeatability of the meters after being treated to extreme humidity, 93±3.0% RH at 89.6 °F, fall within the acceptance criteria. That is, the meters could stand the extreme humidity (93±3% RH, 89.6 °F) as required in EN ISO 15197:2015.

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Image /page/13/Picture/0 description: The image shows the word "bioland" in blue, followed by Chinese characters. The word "bioland" is in lowercase letters, and the Chinese characters are in a similar blue color. There is a registered trademark symbol next to the word "bioland" and the Chinese characters. The text is likely a logo or brand name.

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

有 爱

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen. Guangdong. People's Republic of China

. Vibration and Shock Test

The study was performed in accordance with guideline of IEC 60068-2-64:2008, IEC 61010-1:2010 and EN ISO 15197:2015, Section 5.10: Mechanical resistance to vibration and shock. According to the test results, the function and repeatability after vibration and drop tests fall within the acceptance criteria. That is, the meters could stand the vibration and drop tests required in EN ISO 15197:2015, IEC 60068-2-64:2008 and IEC 61010-1:2010.

. Shelf life evaluation:

The study was performed in accordance with guidelines of ISO 23640:2011. Two components were evaluated respectively for Bioland Blood Glucose Test Strips and Bioland Glucose Control Solutions. There are two cases to conduct for each component. One case is to evaluate the stability of first opening vial in real-time study and another case is shelf life evaluation for unopened vial in real-time study. According to the test results, the unopened vials of the Bioland Blood Glucose Test Strips were stable for 24 months and 90 days for in-use (opened) vials. The unopened vials of the Bioland Glucose Control Solutions were stable for 12 months and 90 days for in-use (opened) vials.

. Sample perturbation study:

Sample perturbation occurs when a user has applied an appropriate volume of blood to the test strip for glucose measurement but an event such as wicking of blood away from the test strip, flicking of the test strip or flicking of the meter occurs during the start of measurement. Under the FDA Guideline "Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use" issued on October 11, 2016 and CLSI EP7-A2, the results show the sampling perturbation has no significant effect on precise blood glucose measurement for Bioland Blood Glucose Monitoring System.

. Testing with used strips:

Under the directions of FDA Guideline "Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use" issued on October 11, 2016, the test results showed when the Bioland Blood Glucose Monitoring System is tested with a used test strip, the System will not provide any measurement data, and the 5-second countdown will not activate.

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Image /page/14/Picture/0 description: The image shows the word "bioland" in blue, followed by three Chinese characters, also in blue. The font is sans-serif and the text is left-aligned. There is a trademark symbol to the right of the word "bioland" and above the Chinese characters.

有 爱 有 快 乐

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen, Guangdong, People's Republic of China

. User performance study (Lay user study):

This User performance study was conducted under the directions of FDA Guideline "Self-Monitoring Blood Glucose Test Systems for Over-the-Counter Use" issued on October 11, 2016. After a certain period of reading user manual, the Bioland Blood Glucose Monitoring System G-427B can be operated properly by a lay user. The test results show that the populations of individual bias ±15% for glucose concentration across the entire range are over 95%. The test results meet the acceptance criteria. We claim that the Bioland Blood Glucose Monitoring System can be operated properly by lay users even in the case of the absence of any guidance.

Summary of data within specified mg/dL of the comparator method for glucose concentrations across the entire range:

Within ±5%Within ±10%Within ±15%Within ±20%
173/351 ( 49.29% )293/351 ( 83.48% )346/351 ( 98.58% )351/351 ( 100% )

. Satisfaction evaluation

More than 85% of volunteers agree that the labels on the packing and the user manual provided enough information of the product for the readers. More than 95 % of volunteers' score were higher than 80 points. It indicates that the written materials could provide enough information of the product for the lay users, so they can comprehend the contents easily even without the explanation from the professionals.

. Usability engineering file:

This usability engineering file is prepared in accordance with EN 60601-1-6: 2010 (IEC 60601-1-6: 2010) and EN 62366: 2008 (IEC 62366: 2007). It contains the records/documents generated for usability engineering process activities. After reviewing the records of usability validation activities, evidence shows that there is no usability problem when validated under normal condition and the worst case scenarios.

  • . Virucidal efficacy against Hepatitis B surface Antigen (HBsAg) by the Clorox Germicidal Wipes disinfectant is conducted on the following parts:
    • On main housing materials of ABS,
    • On test slot (port) of PMMA (transparent), and
    • On LCD display lens of PC (transparent).

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Image /page/15/Picture/0 description: The image shows the word "bioland" in blue, followed by three Chinese characters that also appear in blue. There is a registered trademark symbol to the upper right of the "d" in "bioland", and another registered trademark symbol to the upper right of the last Chinese character. The font is sans-serif and the overall design is simple and clean.

Telephone: +86 755 2896 1218 Facsimile: +86 755 2896 2868 Website: http:// www.bioland.com.cn

Bioland Technology Ltd.

No. A6B7 (Block G), ShangRong Industrial Zone No.5 Baolong Road, Baolong Community, Longgang District 518116 Shenzhen, Guangdong, People's Republic of China

The results passed the acceptance criteria for three parts of the subject device.

● Conclusions

The conclusions drawn from the non-clinical and clinical tests demonstrate that the device is as safe, as effective, and performs as well as the legally marketed device identified in the submission. Thus, the subject device is substantially equivalent to the predicate device.

§ 862.1345 Glucose test system.

(a)
Identification. A glucose test system is a device intended to measure glucose quantitatively in blood and other body fluids. Glucose measurements are used in the diagnosis and treatment of carbohydrate metabolism disorders including diabetes mellitus, neonatal hypoglycemia, and idiopathic hypoglycemia, and of pancreatic islet cell carcinoma.(b)
Classification. Class II (special controls). The device, when it is solely intended for use as a drink to test glucose tolerance, is exempt from the premarket notification procedures in subpart E of part 807 of this chapter subject to the limitations in § 862.9.