K Number
K962441
Device Name
PROSPORE(STEARO FOR STEAM STERILIZER)
Date Cleared
1996-09-18

(86 days)

Product Code
Regulation Number
880.2800
AI/MLSaMDIVD (In Vitro Diagnostic)TherapeuticDiagnosticis PCCP AuthorizedThirdparty
Intended Use
used for determining the efficiency of a 121°C steam sterilization cycle.
Device Description
ProSpore® is a self-contained biological indicator used for determining the efficiency of a 121°C steam sterilization cycle. The device is a Bacillus stearothermophilus in a recovery medium of Tryptic Soy Broth with Bromocresol purple, a pH indicator.
More Information

Not Found

No
The device description and performance studies focus on biological and chemical indicators, with no mention of AI or ML.

No
The device is used to determine the efficiency of a sterilization cycle, not to treat a medical condition.

No
Explanation: The device is a biological indicator used to test the efficiency of a sterilization cycle, not to diagnose a medical condition in a patient.

No

The device description clearly states it is a "self-contained biological indicator" and describes physical components like Bacillus stearothermophilus and a recovery medium. This indicates a physical device, not software only.

Based on the provided information, this device is not an IVD (In Vitro Diagnostic).

Here's why:

  • Intended Use: The intended use is "for determining the efficiency of a 121°C steam sterilization cycle." This is a quality control measure for a sterilization process, not a diagnostic test performed on a biological sample from a human or animal to diagnose a disease or condition.
  • Device Description: The device contains Bacillus stearothermophilus spores and a growth medium. It's designed to assess the effectiveness of sterilization by seeing if the spores are killed, not to analyze a patient sample.
  • Lack of Patient Information: There is no mention of an anatomical site, patient age range, or intended user/care setting related to patient diagnosis or treatment.
  • Performance Studies: The performance studies focus on the device's ability to indicate sterilization effectiveness (incubation period, spore recovery, stability), not on diagnostic accuracy metrics like sensitivity, specificity, etc.
  • Predicate Devices: The predicate devices listed are also biological indicators for sterilization, not IVDs.

In summary, the device's purpose is to evaluate the effectiveness of a sterilization process, which falls under quality control for medical devices or procedures, not in vitro diagnostics.

N/A

Intended Use / Indications for Use

ProSpore® is a self-contained biological indicator used for determining the efficiency of a 121°C steam sterilization cycle.

Product codes

Not Found

Device Description

ProSpore® is a self-contained biological indicator used for determining the efficiency of a 121°C steam sterilization cycle. The device is a Bacillus stearothermophilus in a recovery medium of Tryptic Soy Broth with Bromocresol purple, a pH indicator.

Mentions image processing

Not Found

Mentions AI, DNN, or ML

Not Found

Input Imaging Modality

Not Found

Anatomical Site

Not Found

Indicated Patient Age Range

Not Found

Intended User / Care Setting

Not Found

Description of the training set, sample size, data source, and annotation protocol

Not Found

Description of the test set, sample size, data source, and annotation protocol

Not Found

Summary of Performance Studies

Testing was performed to validate the labeled claims and performance characteristics for ProSpore®. These included studies on the labeled incubation period, recovery of damaged spores, the effect of the pH indicator on the recovery of injured spores, and the stability of population and D-value after a real time of one year. Test results confirmed that the tested samples conformed to the labeled claims.

Key Metrics

Not Found

Predicate Device(s)

K905425

Reference Device(s)

Not Found

Predetermined Change Control Plan (PCCP) - All Relevant Information

Not Found

§ 880.2800 Sterilization process indicator.

(a)
Biological sterilization process indicator —(1)Identification. A biological sterilization process indicator is a device intended for use by a health care provider to accompany products being sterilized through a sterilization procedure and to monitor adequacy of sterilization. The device consists of a known number of microorganisms, of known resistance to the mode of sterilization, in or on a carrier and enclosed in a protective package. Subsequent growth or failure of the microorganisms to grow under suitable conditions indicates the adequacy of sterilization.(2)
Classification. Class II (performance standards).(b)
Physical/chemical sterilization process indicator —(1)Identification. A physical/chemical sterilization process indicator is a device intended for use by a health care provider to accompany products being sterilized through a sterilization procedure and to monitor one or more parameters of the sterilization process. The adequacy of the sterilization conditions as measured by these parameters is indicated by a visible change in the device.(2)
Classification. Class II (performance standards).

0

K962441

Summary of "ProSpore Self-contained Biological Indicator" for steam sterilization at 121ºC Submitter: Raven Biological Laboratories, Inc.

5017 Leavenworth Street Omaha, NE 68106

Production Microbiologist

(402) 556-6690 (402) 556-4722 FAX

Russ Nyberg

20 June 1996

Kilt™ (BBL)

Contact:

Prepared on:

Device name:

Classification:

.

Predicate Devices (legally marketed)

Predicate Devices 510(k) number:

Kilit™(BBL) - Grandfather In* Spor View™ (SPSmedical) K905425

Spor View™ (SPSmedical)

ProSpore® self-contained biological indicator

Class II medical device, General Hospital

Description

ProSpore® is a self-contained biological indicator used for determining the efficiency of a 121°C steam sterilization cycle. The device is a Bacillus stearothermophilus in a recovery medium of Tryptic Soy Broth with Bromocresol purple, a pH indicator.

Specifications

  1. BI

Bacillus stearothermophilus (ATCC # 7953) Microorganism:

Population: 1.0 x 105 - 4.0 x 105 cfu/unit

Resistance Characteristics: (for saturated steam at 121ºC)

1

D-value: (determined using Spearman - Karber method on pages 43-50).

| Survival time: | (USP XXII, p. 204 - Attachment #9)
=[min] labeled D-value x (log10 labeled spore count per carrier -2) |
|----------------|------------------------------------------------------------------------------------------------------------|
| Kill time: | (USP XXII, p. 204 - Attachment #9))
=[max] labeled D-value x (log10 labeled spore count per carrier +4) |

Incubation period: 77 days

2) Components

Ampule: USP type 1. 4-ml flame-seal flint glass ampule

Culture/recovery medium: 10 ml of Bromocresol Purple (BCP) stock solution to each liter of Trypticase® Soy Broth (TSB), adjusted to a pH of 7.0 with Sodium Hydroxide (NaOH) and/or Hydrochloric Acid (HCL

  • Trypticase® Soy Broth powder (Becton Dickinson), as per manufacturer's nutrient: instructions
    neutralizers: Sodium Hydroxide (NaOH) and Hydrochloric Acid (HCL) pH indicator: Bromocresol Purple (4 grams BCP powder to each liter of deionized distilled water)

Operational Principles

The ProSpore® ampule is placed with a load in the sterilization chamber, and subjected to a normal steam sterilization cycle. The ampule is then removed and cooled to room temperature. Next, the processed ampule and an unprocessed (control) ampule are placed in incubation for a period of 7 days at a temperature favorable for growth (55° - 60° C. for B. stearothermophilus).

During incubation, the available food supply (TSB) and temperature promote growth of viable spore. The growth process may be accompanied by turbidity and/or a release of acidic waste by products which reduce the pH level of the surrounding medium. Bromocresol purple reacts to this reduction by changing in color to or toward yellow

Within 7 days, growth will become evident by a change in color from purple to/toward yellow and/or turbidity in the test ampule. This may be interpreted as a failure to meet the conditions necessary for sterilization, provided these signs are present in the control ampule.

Statement of similarity to legally marketed (predicate) devices:

ProSpore® is similar in composition and function to Kilt™ and SporView™, as these devices, like ProSpore® combines Bacillus stearothermophilus spores and a recovery medium (combined

2

with a pH indicator) in a flame-sealed glass ampule for use in determining the efficacy of steam sterilization cycles.

Clinical Tests: No clinical tests have been performed.

Discussion of results from nonclinical tests:

Testing was performed to validate the labeled claims and performance characteristics for ProSpore®. These included studies on the labeled incubation period, recovery of damaged spores, the effect of the pH indicator on the recovery of injured spores, and the stability of population and D-value after a real time of one year. Test results confirmed that the tested samples conformed to the labeled claims.

Statement of safety and effectiveness

Based on similar claims and design, and results from the nonclinical studies mentioned above, the ProSpore® biological indicator has been determined to be substantially equivalent and, therefore, as safe and effective to the legally marketed devices Kilt™ and Spor View™

*Kilit™(BBL) does not have a 510 (k) number. It was marketed under the grandfather clause, which exempts devices in commercial distribution prior to May 28, 1976. We have obtained the results of the Trademarkscan search of the U.S. Patent Office trademark filing showing that the product was introduced into commerce on June 8, 1955 (Attachment 10). The FDA # is M452998