K Number
K013278
Device Name
TINA-QUANT APOLIPOPROTEIN A-1 VER.2
Date Cleared
2001-11-15

(45 days)

Product Code
Regulation Number
866.5590
AI/MLSaMDIVD (In Vitro Diagnostic)TherapeuticDiagnosticis PCCP AuthorizedThirdparty
Intended Use
In vitro diagnostic reagent system intended for use on COBAS INTEGRA system for the quantitative immunological determination of human apolipoprotein A-1 in serum and plasma. For the quantitative determination of apolipoprotein A-1 in serum and plasma. A lipoprotein test system is a device intended to measure lipoprotein in serum and plasma. Lipoprotein measurements are used in the diagnosis and treatment of lipid disorders and atherosclerosis.
Device Description
Human apolipoprotein A-1 forms a precipitate with a specific antiserum which is determined turbidimetrically at 340 nm.
More Information

No
The description focuses on a turbidimetric assay for quantitative determination of apolipoprotein A-1, a standard laboratory technique, with no mention of AI or ML.

No.
The device is an in vitro diagnostic reagent system used for measuring human apolipoprotein A-1 in serum and plasma, which is for diagnostic purposes, not for treating a disease or condition.

Yes
The intended use explicitly states, "In vitro diagnostic reagent system..." and "Lipoprotein measurements are used in the diagnosis and treatment of lipid disorders and atherosclerosis."

No

The device description clearly states it is an "in vitro diagnostic reagent system" and describes a turbidimetric measurement method, indicating it involves physical reagents and a hardware-based measurement system (COBAS INTEGRA system). This is not a software-only device.

Yes, this device is an IVD (In Vitro Diagnostic).

Here's why:

  • Intended Use/Indications for Use: The very first sentence explicitly states "In vitro diagnostic reagent system intended for use on COBAS INTEGRA system for the quantitative immunological determination of human apolipoprotein A-1 in serum and plasma." This directly identifies it as an IVD.
  • Nature of the Test: The device measures a substance (apolipoprotein A-1) in biological samples (serum and plasma) to aid in the diagnosis and treatment of medical conditions (lipid disorders and atherosclerosis). This is the core function of an IVD.
  • Device Description: The description details a method for analyzing a biological sample (turbidimetric determination of a precipitate formed with antiserum). This is consistent with IVD technology.

N/A

Intended Use / Indications for Use

In vitro diagnostic reagent system intended for use on COBAS INTEGRA system for the quantitative immunological determination of human apolipoprotein A-1 in serum and plasma.

For the quantitative determination of apolipoprotein A-1 in serum and plasma. A lipoprotein test system is a device intended to measure lipoprotein in serum and plasma. Lipoprotein measurements are used in the diagnosis and treatment of lipid disorders and atherosclerosis.

Product codes

DER

Device Description

Human apolipoprotein A-1 forms a precipitate with a specific antiserum which is determined turbidimetrically at 340 nm.

Mentions image processing

Not Found

Mentions AI, DNN, or ML

Not Found

Input Imaging Modality

Not Found

Anatomical Site

Not Found

Indicated Patient Age Range

Not Found

Intended User / Care Setting

Not Found

Description of the training set, sample size, data source, and annotation protocol

Not Found

Description of the test set, sample size, data source, and annotation protocol

Not Found

Summary of Performance Studies

The performance characteristics of the Tina-quant Apolipoprotein A-1 ver.2 are compared to the predicate device in a table.

Precision:

  • Tina-quant Apolipoprotein A-1 ver.2:
    • Within run CV: 1.0% @ 0.88 g/L; 0.8% @ 1.64 g/L
    • Between Day CV: 2.4% @ 0.88 g/L; 1.7% @ 1.64 g/L
  • Apolipoprotein A-1 (predicate):
    • Within run CV: 1.5% @ 0.68 g/L; 1.0% @ 2.7 g/L
    • Between Day CV: 1.2% @ 0.68g/L; 0.78% @ 2.7 g/L

Method Comparison (Bablok/Passing):

  • Tina-quant Apolipoprotein A-1 ver.2 (Y) / COBAS Integra Apolipoprotein A-1 (X): y = 0.87x + 0.25 g/L, r = 0.940
  • Apolipoprotein A-1 (Y) / commercially available system (X): y = 1.19x- 0.2 g/L, r = 0.993

Prozone Effect:

  • Tina-quant Apolipoprotein A-1 ver.2: >6 g/L
  • Apolipoprotein A-1 (predicate): >5.8g/L

Analytical sensitivity (LDL):

  • Tina-quant Apolipoprotein A-1 ver.2: 0.058 g/L (5.8 mg/dL)
  • Apolipoprotein A-1 (predicate): 0.37 g/L (37mg/dL)

Limitations of both devices:

  • Icterus: No significant interference
  • Hemolysis: No significant interference
  • Lipemia: No significant interference (up to 1000 mg/dL Intralipid for Tina-quant Apolipoprotein A-1 ver.2)
  • Rheumatoid factors: No significant interference

Key Metrics (Sensitivity, Specificity, PPV, NPV, etc.)

Not Found

Predicate Device(s)

K990594

Reference Device(s)

Not Found

Predetermined Change Control Plan (PCCP) - All Relevant Information

Not Found

§ 866.5590 Lipoprotein X immunological test system.

(a)
Identification. A lipoprotein X immunological test system is a device that consists of the reagents used to measure by immunochemical techniques lipoprotein X (a high-density lipoprotein) in serum and other body fluids. Measurement of lipoprotein X aids in the diagnosis of obstructive liver disease.(b)
Classification. Class I (general controls). The device is exempt from the premarket notification procedures in subpart E of part 807 of this chapter subject to § 866.9.

0

NOV 1 5 2001

013278

| Introduction | According to the requirements of 21 CFR 807.92, the following information
provides sufficient detail to understand the basis for a determination of
substantial equivalence. |
|----------------------------------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| Submitter
name, address,
contact | Roche Diagnostics Corporation
9115 Hague Road
Indianapolis, IN 46250
(317) 521-7643 |
| | Contact Person: Helen T. Torney |
| | Date Prepared: September 27, 2001 |
| Device Name | Proprietary name: Tina-quant Apolipoprotein A-1 ver.2 |
| | Common name: Apolipoprotein A-1 |
| | Classification name: Alpha-1- lipoprotein immunological test system |
| Device
Description | Human apolipoprotein A-1 forms a precipitate with a specific antiserum
which is determined turbidimetrically at 340 nm. |
| Intended use | In vitro diagnostic reagent system intended for use on COBAS INTEGRA
system for the quantitative immunological determination of human
apolipoprotein A-1 in serum and plasma. |
| Indications for
Use | For the quantitative determination of apolipoprotein A-1 in serum and
plasma. A lipoprotein test system is a device intended to measure lipoprotein
in serum and plasma. Lipoprotein measurements are used in the diagnosis
and treatment of lipid disorders and atherosclerosis. |

510(k) Summary

1

510(k) Summary, Continued

Substantial Equivalence

The Tina-quant Apolipoprotein A-1 ver.2 is substantially equivalent to other devices legally marketed in the United States. We claim equivalence to the COBAS Integra Tina-quant Apolipoprotein A-1 (K990594).

Substantial equivalence similarities

The following table compares the Tina-quant Apolipoprotein A-1 ver.2 Assay with the predicate device.

| Feature | Tina-quant
Apolipoprotein A-1 ver.2 | Apolipoprotein A-1
(predicate) |
|--------------------------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| Intended Use | In vitro diagnostic reagent system intended for use on COBAS INTEGRA system for the quantitative immunological determination of human apolipoprotein A-1 in serum and plasma. | In vitro diagnostic reagent system intended for use on COBAS Integra (analyzer model) for the quantitative immunological determination of human apolipoprotein A-1 in serum and plasma. |
| Indication for Use | For the quantitative determination of apolipoprotein A-1 in serum and plasma. A lipoprotein test system is a device intended to measure lipoprotein in serum and plasma. Lipoprotein measurements are used in the diagnosis and treatment of lipid disorders and atherosclerosis. | For the quantitative determination of apolipoprotein A-1 in serum and plasma. A lipoprotein test system is a device intended to measure lipoprotein in serum and plasma. Lipoprotein measurements are used in the diagnosis and treatment of lipid disorders and atherosclerosis. |
| Assay Protocol | Immunoturbidimetric | Immunoturbidimetric |
| Instrument | COBAS Integra Clinical Chemistry Analyzers | COBAS Integra Clinical Chemistry Analyzers |
| Traceability / Standardization | Standardized with regard to the WHO/IFCC reference material SP1-01. | Standardized with regard to the WHO/IFCC reference material SP1-01. |
| Sample Type | Serum and plasma | Serum and plasma |

2

510(k) Summary, Continued

| Feature | Tina-quant Apolipoprotein
A-1 ver.2 | Apolipoprotein A-1
(predicate) |
|-------------------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| Calibration
Interval | After each lot | After each lot |
| Formulation | R: TRIS buffer,
polyethylene glycol.
detergent, preservative
(liquid).
SR: Anti-apolipoprotein A-1
antibody (sheep) specific for
human Apo A-1, TRIS
buffer, preservative (liquid). | R: Anti-apolipoprotein A-1
T antiserum (rabbit) specific
for human Apo A-1, in
phosphate buffer stabilized
with 0.09% sodium azide in
vial A (liquid). |

Substantial equivalence differences

The following table compares the Tina-quant Apolipoprotein B ver.2 Assay with the predicate device.

| Feature | Tina-quant Apolipoprotein
A-1 ver.2 | Apolipoprotein A-1
(predicate) |
|----------------------|----------------------------------------|-----------------------------------|
| Reagent
Stability | On board: 4 weeks | On-board: 12 weeks |
| Calibrator | C.f.a.s. Lipids | Apolipoprotein T Standard |
| Controls | Precinorm L, Precipath L | Apolipoprotein T Control |
| Expected | Females:1.08-2.25 g/L | Females: 1.10-2.05g/L |
| Values | Males: 1.04-2.02 g/L | Males: 1.10-1.80 g/L |
| Measuring | 0.20 - 4.0 g/L | 0.37-4.0 g/L |
| Range | (0.10 - 4.0 g/L with rerun) | (0.12 - 5.6 g/L with rerun) |

3

510(k) Summary, Continued

Substantial equivalence performance characteristics The performance characteristics of the Tina-quant Apolipoprotein A-1 ver.2 I he performance characterice are compared in the table below.

| Feature | Tina-quant Apolipoprotein
A-1 ver.2 | Apolipoprotein A-1
(predicate) |
|---------------------------------|--------------------------------------------------------------------------------------------------------------------------------------------------|------------------------------------------------------------------------------------------------------------------------|
| Precision | Within run CV
1.0% @ 0.88 g/L
0.8% @ 1.64 g/L
Between Day CV
2.4% @ 0.88 g/L
1.7% @ 1.64 g/L | Within run CV
1.5% @ 0.68 g/L
1.0% @ 2.7 g/L
Between Day CV
1.2% @ 0.68g/L
0.78% @ 2.7 g/L |
| Method
Comparison | Bablok/Passing:
Tina-quant Apolipoprotein
A-1 ver.2 (Y) / COBAS
Integra Apolipoprotein A-1
(X).
y = 0.87x + 0.25 g/L
r = 0.940 | Bablok/Passing:
Apolipoprotein A-1 (Y)/
commercially available
system (X).
y = 1.19x- 0.2 g/L
r = 0.993 |
| Prozone Effect | >6 g/L | >5.8g/L |
| Analytical
sensitivity (LDL) | 0.058 g/L (5.8 mg/dL) | 0.37 g/L (37mg/dL) |

4

Premarket Notification, 510(k) for Tina-quant Apolipoprotein A-1 ver. 2 Test System on COBAS Integra Clinical Chemistry

Analyzers, continued

Substantial equivalence performance characteristics, cont.

The performance characteristics of the Tina-quant Apolipoprotein A-1 ver.2 Assay and the predicate device are compared in the table below.

| Feature | Tina-quant Apolipoprotein
A-1 ver.2 | Apolipoprotein A-1 |
|-------------|---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| Limitations | • Icterus: No significant
interference
• Hemolysis: No
significant interference
• Lipemia: No significant
interference up to an
Intralipid level of 1000
mg/dL
• Rheumatoid factors: No
significant interference | • Icterus: No significant
interference
• Hemolysis: No
significant
interference
• Lipemia: No
significant
interference
• Rheumatoid factors:
No significant
interference |

5

Image /page/5/Picture/0 description: The image shows the logo for the U.S. Department of Health & Human Services. The logo consists of a stylized depiction of an eagle or bird-like figure with three horizontal lines extending from its head, resembling wings or feathers. The text "DEPARTMENT OF HEALTH & HUMAN SERVICES - USA" is arranged in a circular fashion around the emblem.

Food and Drug Administration 2098 Gaither Road Rockville MD 20850

Ms. Helen Torney Regulatory Submissions, Centralized Diagnostics Roche Diagnostics Corporation 9115 Hague Road P.O. Box 50457 Indianapolis, IN 46250-0457

Re: K013278

Trade/Device Name: Tina-quant Apolipoprotein A-1 ver.2 Regulation Number: 21 CFR 866.5580 Regulation Name: Alpha-1-lipoprotein immunological test system. Regulatory Class: Class II Product Code: DER Dated: September 27, 2001 Received: October 1, 2001

Dear Ms. Torney:

We have reviewed your Section 510(k) premarket notification of intent to market the device we nave reviewed your betermined the device is substantially equivalent (for the indications for use stated in the enclosure) to legally marketed predicate devices marketed in interstate for ass stated in the encreated of 76 the enactment date of the Medical Device Amendments, or to devices that have been reclassified in accordance with the provisions of the Federal Food, Drug, de frees that have been require approval of a premarket approval application (PMA). and Costietle Fror (110) that the device, subject to the general controls provisions of the Act. The r ou may ; iferences mixes of the Act include requirements for annual registration, listing of devices, good manufacturing practice, labeling, and prohibitions against misbranding and adulteration.

NOV 1 5 2001

If your device is classified (see above) into either class II (Special Controls) or class III (PMA), it If your device is classinod (soo as controls. Existing major regulations affecting your device can may be subject to back adder addess, Title 21, Parts 800 to 898. In addition, FDA may publish further announcements concerning your device in the Federal Register.

Please be advised that FDA's issuance of a substantial equivalence determination does not mean that FDA has made a determination that your device complies with other requirements of the Act that I Dri has made a and regulations administered by other Federal agencies. You must or any I oderal blacked and st requirements, including, but not limited to: registration and listing (21 CFR Part 807); labeling (21 CFR Part 801); good manufacturing practice requirements as set forth in the quality systems (21 CFR Part 820); and if applicable, the electronic product radiation control provisions (Sections 531-542 of the Act); 21 CFR 1000-1050.

6

Page 2 -

This letter will allow you to begin marketing your device as described in your 510(k) premarket notification. The FDA finding of substantial equivalence of your device to a legally marketed nothleation. The I Dri Imaling of castin for your device and thus, permits your device to proceed to the market.

If you desire specific advice for your device on our labeling regulation (21 CFR Part 801 and 1 II you desire specific articles from astic devices), please contact the Office of Compliance at additionally 607.10 for mile ar questions on the promotion and advertising of your device, (201) 594-1566. Tracksonally (194-4639. Also, please note the regulation entitled, "Misbranding by reference to premarket notification" (21CFR 807.97). Other general information on your responsibilities under the Act may be obtained from the Division of Small Information on your responsibilities are at its toll-free number (800) 638-2041 or Manufacturers International met address "http://www.fda.gov/cdrb/dsma/dsmamain.html".

Sincerely yours,

Steven Putman

Steven I. Gutman, M.D., M.B.A. Director Division of Clinical Laboratory-Devices Office of Device Evaluation Center for Devices and Radiological Health

Enclosure

7

KD13278

Indications for Use Statement

NOV 1 5 2001

510(k) Number (if known): N/A

Device Name: Tina-quant Apolipoprotein A-1 ver.2

Indications For Use:

In vitro diagnostic reagent system intended for use on COBAS INTEGRA system for the quantitative immunological determination of human apolipoprotein A-1 in serum and plasma.

For the quantitative determination of apolipoprotein A-1 in serum and plasma. A lipoprotein test system is a device intended to measure lipoprotein in serum and plasma. Lipoprotein measurements are used in the diagnosis and treatment of lipid disorders and atherosclerosis.

Prescription Use
(Per 21 CFR 801.109)

OR

Over-The-Counter Use

(Optional Format 1-2-96)

Looges

(Divisio -Off)
Division: Lanical Laboratory Devices
510(k) Number: K013278