K Number
K012357
Device Name
CA 15-3 ASSAY FOR THE ADVIA CENTAUR SYSTEM
Date Cleared
2002-02-28

(218 days)

Product Code
Regulation Number
866.6010
AI/MLSaMDIVD (In Vitro Diagnostic)TherapeuticDiagnosticis PCCP Authorized
Intended Use
The ADVIA Centaur CA 15-3 assay is an in vitro diagnostic test for the quantitative serial determination of cancer antigen CA 15-3 in human serum using the ADVIA Centaur® System. When used in conjunction with other clinical and diagnostic procedures, serial testing with the ADVIA Centaur CA 15-3 assay is useful for monitoring the course of disease and therapy in metastatic breast cancer patients, and for detection of recurrence in previously treated patients with Stage II, with greater than two positive lymph nodes, or Stage III breast cancer patients. This assay is not intended for use on any other system.
Device Description
The ADVIA Centaur CA 15-3 assay is a fully automated, two-step sandwich immunoassay using direct, chemiluminescent technology. The Lite Reagent is composed of the monoclonal mouse antibody, DF3, specific for CA 15-3, labeled with acridinium ester. The Conjugate Reagent is composed of the monoclonal mouse antibody 115D8, specific for CA 15-3, labeled with fluorescein. The Solid Phase is composed of purified monoclonal mouse capture antibody, which is covalently coupled to paramagnetic particles. The sample is incubated with both Conjugate Reagent and Solid Phase simultaneously for 20 minutes. After incubation, the immuno-complex is washed and the Lite Reagent is added, incubated for an additional 20 minutes and then washed again. The measured chemiluminescence is directly proportional to the quantity of CA 15-3 antigen in the sample.
More Information

No
The description details a standard immunoassay technology and does not mention any AI or ML components.

No
The device is an in vitro diagnostic test used for monitoring disease and therapy, not for providing therapy itself.

Yes
The "Intended Use / Indications for Use" section explicitly states that the device is "an in vitro diagnostic test for the quantitative serial determination of cancer antigen CA 15-3 in human serum." It also mentions its use for "monitoring the course of disease and therapy in metastatic breast cancer patients, and for detection of recurrence."

No

The device description clearly outlines physical components (reagents, solid phase, paramagnetic particles) and a chemical process (immunoassay, chemiluminescence), indicating it is a hardware-based in vitro diagnostic device, not software-only.

Yes, this device is an IVD (In Vitro Diagnostic).

The "Intended Use / Indications for Use" section explicitly states: "The ADVIA Centaur CA 15-3 assay is an in vitro diagnostic test for the quantitative serial determination of cancer antigen CA 15-3 in human serum using the ADVIA Centaur® System."

This statement directly identifies the device as an in vitro diagnostic test.

N/A

Intended Use / Indications for Use

The ADVIA Centaur CA 15-3 assay is an in vitro diagnostic test for the quantitative serial determination of cancer antigen CA 15-3 in human serum using the ADVIA Centaur® System. When used in conjunction with other clinical and diagnostic procedures, serial testing with the ADVIA Centaur CA 15-3 assay is useful for monitoring the course of disease and therapy in metastatic breast cancer patients, and for detection of recurrence in previously treated patients with Stage II, with greater than two positive lymph nodes, or Stage III breast cancer patients. This assay is not intended for use on any other system.

Product codes (comma separated list FDA assigned to the subject device)

82 MOI

Device Description

The ADVIA Centaur CA 15-3 assay is a fully automated, two-step sandwich immunoassay using direct, chemiluminescent technology. The Lite Reagent is composed of the monoclonal mouse antibody, DF3, specific for CA 15-3, labeled with acridinium ester. The Conjugate Reagent is composed of the monoclonal mouse antibody 115D8, specific for CA 15-3, labeled with fluorescein. The Solid Phase is composed of purified monoclonal mouse capture antibody, which is covalently coupled to paramagnetic particles. The sample is incubated with both Conjugate Reagent and Solid Phase simultaneously for 20 minutes. After incubation, the immuno-complex is washed and the Lite Reagent is added, incubated for an additional 20 minutes and then washed again. The measured chemiluminescence is directly proportional to the quantity of CA 15-3 antigen in the sample.

Mentions image processing

Not Found

Mentions AI, DNN, or ML

Not Found

Input Imaging Modality

Not Found

Anatomical Site

Not Found

Indicated Patient Age Range

Not Found

Intended User / Care Setting

Not Found

Description of the training set, sample size, data source, and annotation protocol

Not Found

Description of the test set, sample size, data source, and annotation protocol

Not Found

Summary of Performance Studies (study type, sample size, AUC, MRMC, standalone performance, key results)

For 128 samples in the range of 4.0 to 188.6 U/mL, the relationship of the ADVIA Centaur CA 15-3 assay to the Immuno 1™ CA 15-3 assay is described by the following equation (calculated using Deming Regression):

ADVIA Centaur CA 15-3 = 1.018 (Immuno 1) – 4.0 U/mL

The data demonstrate substantial equivalence of the ADVIA Centaur CA 15-3 assay to the FDA-cleared Baver Immuno-1 CA 15-3 assay as an adjunctive test for use in the management (monitoring) of metastatic breast cancer patients during the course of disease and therapy and for the detection of disease recurrence in Stage II, with greater than two positive lymph nodes, or Stage III breast cancer patients.

Key Metrics (Sensitivity, Specificity, PPV, NPV, etc.)

Not Found

Predicate Device(s): If the device was cleared using the 510(k) pathway, identify the Predicate Device(s) K/DEN number used to claim substantial equivalence and list them here in a comma separated list exactly as they appear in the text. List the primary predicate first in the list.

K964703

Reference Device(s): Identify the Reference Device(s) K/DEN number and list them here in a comma separated list exactly as they appear in the text.

Not Found

Predetermined Change Control Plan (PCCP) - All Relevant Information for the subject device only (e.g. presence / absence, what scope was granted / cleared under the PCCP, any restrictions, etc).

Not Found

§ 866.6010 Tumor-associated antigen immunological test system.

(a)
Identification. A tumor-associated antigen immunological test system is a device that consists of reagents used to qualitatively or quantitatively measure, by immunochemical techniques, tumor-associated antigens in serum, plasma, urine, or other body fluids. This device is intended as an aid in monitoring patients for disease progress or response to therapy or for the detection of recurrent or residual disease.(b)
Classification. Class II (special controls). Tumor markers must comply with the following special controls: (1) A guidance document entitled “Guidance Document for the Submission of Tumor Associated Antigen Premarket Notifications (510(k)s) to FDA,” and (2) voluntary assay performance standards issued by the National Committee on Clinical Laboratory Standards.

0

Premarket Notification - 510(k)

FEB 2 8 2002

ADVIA Centaur CA15-3 Immunoassay

510(k) Summary of Safety and Effectiveness

This summary of safety and effectiveness is being submitted in accordance with the requirements of The Safe Medical Devices Act of 1990 (SMDA 1990) and 21 CFR 807.92.

The assigned 510(k) number is: K012357

7.1 General Information

Date of Summary Update: December 11, 2001

| Applicant: | Kenneth T. Edds, Ph.D.
Manager, Regulatory Affairs
Business Group Diagnostics
511 Benedict Ave.
Tarrytown, NY 10591
Phone: 914-524-2446
Fax: 914-524-2500
Ken.edds.b@bayer.com |
|-------------------------------------------------------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| Owner: | Bayer Corporation
Business Group Diagnostics
511 Benedict Ave.
Tarrytown, NY 10591
Establishment Registration No: 2432235 |
| Manufacturer: | Bayer Corporation
333 Coney Street
Walpole, MA 02032
Establishment Registration No: 1219913 |
| Device Name: | ADVIA Centaur® CA 15-3 assay |
| Common or Usual Name: | Chemiluminescence immunoassay kit for the
determination of CA 15-3 antigen
using Bayer Corporation's
ADVIA Centaur automated analyzer. |
| Classification:
Name:
Class:
CFR:
Product Code: | Bayer ADVIA Centaur CA 15-3 Assay
ll
21 CFR 866.6010
82 MOI |

This submission was prepared in accordance with "Guidance Document for Submission of Tumor Associated Antigen Premarket Notifications".

Substantial Equivalence To: Bayer Immuno-1 CA 15-3

K964703 510(k) Number:

1

7.2 Intended Use

The ADVIA Centaur CA 15-3 assay is an in vitro diagnostic test for the quantitative serial determination of cancer antigen CA 15-3 in human serum using the ADVIA Centaur® System. When used in conjunction with other clinical and diagnostic procedures, serial testing with the ADVIA Centaur CA 15-3 assay is useful for monitoring the course of disease and therapy in metastatic breast cancer patients, and for detection of recurrence in previously treated patients with Stage II, with greater than two positive lymph nodes, or Stage III breast cancer patients. This assay is not intended for use on any other system.

7.3 Device Description

The ADVIA Centaur CA 15-3 assay is a fully automated, two-step sandwich immunoassay using direct, chemiluminescent technology. The Lite Reagent is composed of the monoclonal mouse antibody, DF3, specific for CA 15-3, labeled with acridinium ester. The Conjugate Reagent is composed of the monoclonal mouse antibody 115D8, specific for CA 15-3, labeled with fluorescein. The Solid Phase is composed of purified monoclonal mouse capture antibody, which is covalently coupled to paramagnetic particles. The sample is incubated with both Conjugate Reagent and Solid Phase simultaneously for 20 minutes. After incubation, the immuno-complex is washed and the Lite Reagent is added, incubated for an additional 20 minutes and then washed again. The measured chemiluminescence is directly proportional to the quantity of CA 15-3 antigen in the sample.

7.4 Comparison to the Predicate Device

The ADVIA Centaur CA 15-3 immunoassay kit is similar to the Immuno-1 CA15-3 kit in the indications for use, format, performance characteristics, and results. The ADVIA Centaur tests differ mainly in their signal system as compared to the Immuno-1 principle. In the ADVIA Centaur method, a chemiluminogenic molecule (acridinium ester) is used to replace the Alkaline Phosphatase signal used in the Immuno-1 assay.

7.5 Equivalence to Predicate Device

For 128 samples in the range of 4.0 to 188.6 U/mL, the relationship of the ADVIA Centaur CA 15-3 assay to the Immuno 1™ CA 15-3 assay is described by the following equation (calculated using Deming Regression):

ADVIA Centaur CA 15-3 = 1.018 (Immuno 1) – 4.0 U/mL

The data demonstrate substantial equivalence of the ADVIA Centaur CA 15-3 assay to the FDA-cleared Baver Immuno-1 CA 15-3 assay as an adjunctive test for use in the management (monitoring) of metastatic breast cancer patients during the course of disease and therapy and for the detection of disease recurrence in Stage II, with greater than two positive lymph nodes, or Stage III breast cancer patients.

2

DEPARTMENT OF HEALTH & HUMAN SERVICES

Image /page/2/Picture/1 description: The image shows the logo for the U.S. Department of Health & Human Services. The logo consists of a stylized caduceus, which is a symbol of medicine, with three lines representing the branches of government. The text "DEPARTMENT OF HEALTH & HUMAN SERVICES - USA" is arranged in a circular pattern around the caduceus.

Food and Drug Administration 2098 Gaither Road Rockville MD 20850

FEB 2 8 2002

Kenneth T. Edds, Ph.D. Manager, Regulatory Affairs Bayer Diagnostics Corporation 511 Benedict Avenue Tarrytown, NY 10591

K012357 Re: Trade/Device Name: ADVIA Centaur® CA 15-3™ Assay Regulation Number: 21 CFR 866.6010 Regulation Name: Tumor Associated Antigen Immunological Test System Regulatory Class: Class II Product Code: MOI Dated: December 12, 2001 Received: December 14, 2001

Dear Dr. Edds:

We have reviewed your Section 510(k) premarket notification of intent to market the device referenced above and have determined the device is substantially equivalent (for the indications for use stated in the enclosure) to legally marketed predicate devices marketed in interstate commerce prior to May 28, 1976, the enactment date of the Medical Device Amendments, or to devices that have been reclassified in accordance with the provisions of the Federal Food, Drug, and Cosmetic Act (Act) that do not require approval of a premarket approval application (PMA). You may, therefore, market the device, subject to the general controls provisions of the Act. The general controls provisions of the Act include requirements for annual registration, listing of devices, good manufacturing practice, labeling, and prohibitions against misbranding and adulteration.

If your device is classified (see above) into either class II (Special Controls) or class III (PMA), it may be subject to such additional controls. Existing major regulations affecting your device can be found in the Code of Federal Regulations, Title 21, Parts 800 to 898. In addition, FDA may publish further announcements concerning your device in the Federal Register.

Please be advised that FDA's issuance of a substantial equivalence determination does not mean that FDA has made a determination that your device complies with other requirements of the Act or any Federal statutes and regulations administered by other Federal agencies. You must comply with all the Act's requirements, including, but not limited to: registration and listing (21 CFR Part 807); labeling (21 CFR Part 801); good manufacturing practice requirements as set forth in the quality systems (QS) regulation (21 CFR Part 820); and if applicable, the electronic product radiation control provisions (Sections 531-542 of the Act); 21 CFR 1000-1050.

3

Page 2 -

This letter will allow you to begin marketing your device as described in your 510(k) premarket notification. The FDA finding of substantial equivalence of your device to a legally marketed predicate device results in a classification for your device and thus, permits your device to proceed to the market.

If you desire specific advice for your device on our labeling regulation (21 CFR Part 801 and ' additionally 809.10 for in vitro diagnostic devices), please contact the Office of Compliance at (301) 594-4588. Additionally, for questions on the promotion and advertising of your device, please contact the Office of Compliance at (301) 594-4639. Also, please note the regulation entitled. "Misbranding by reference to premarket notification" (21CFR 807.97). Other general information on your responsibilities under the Act may be obtained from the Division of Small Manufacturers International and Consumer Assistance at its toll-free number (800) 638-2041 or (301) 443-6597 or at its internet address "http://www.fda.gov/odrh/dsma/dsmamain.html".

Sincerely yours,

Steven Sutman

Steven I. Gutman, M.D., M.B.A. Director Division of Clinical Laboratory Devices Office of Device Evaluation Center for Devices and Radiological Health

Enclosure

4

Page 1 of 1

510(k) Number: Kol 2357

Device Name: CA 15-3 Assay for the Advia Centaur

Indications for Use:

The ADVIA Centaur CA 15-3 assay is an in vitro diagnostic test for the quantitative serial determination of cancer antigen CA 15-3 in human serum using the ADVIA Centaur® System. When used in conjunction with other clinical and diagnostic procedures, serial testing with the ADVIA Centaur CA 15-3 assay is useful for monitoring the course of disease and therapy in metastatic breast cancer patients, and for detection of recurrence in previously treated patients with Stage II, with greater than two positive lymph nodes, or Stage III breast cancer patients. This assay is not intended for use on any other system.

(PLEASE DO NOT WRITE BELOW THIS LINE - CONTINUE ON ANOTHER PAGE IF NEEDED)

Concurrence of CDRH, Office of Device Evaluation (ODE)

Sousan S. Altaie
(Division Sign-Off)

510(k) Number K012357

Prescription Use V (Per 21 CFR 801.109) OR

Over-The-CounterUse