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Affirm 400 is a surgical microscope accessory used in fluorescent visualization of suspected grade III and IV gliomas during neurosurgery, comprising an excitation filter for blue spectral range between 390 nm and 420 nm and an observation filter for visible light with spectral range between 510 nm and 700 nm.

Affirm 400 is an accessory to the Class I Digital Surgical Microscope (DSM). Affirm 400 is a hardware and software which allows intraoperative viewing of suspected grade III and IV gliomas under fluorescence with the 5-ALA optical imaging agent. Affirm 400 is composed of optical filters: the "Excitation" filter and the "Emission" filter. The Excitation filter is designed to filter light between 390-420 nanometers. The Emission filter is designed to filter light between 510 – 700 nanometers. The use of a suitable detection system allows for visualization of surgical interventions. The emitted light is then captured by the optics of the digital microscope, passed through filters to remove unwanted wavelengths of light, and finally detected by the image sensors. This detected signal is then projected on a monitor enabling the surgeon to view the magnified image. The Affirm 400 includes installation of a software license that facilitates use of the accessory. After the software license is installed, the user has the option to switch from the normal white light mode of the surgical microscope to the Affirm 400 mode.

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